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教 授 |
柴田 信之 |
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准 教 授 |
佐々木 雅人 |
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助 手 |
伊藤 文恵、田中 大 |
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薬学科 |
６年 伊藤 悠、菅原隼斗、関根僚也、呂 菜奈恵、酒井 萌、鈴木美貴恵、松本宏美、石川左和子、新目樺子、飯塚さくら |
薬学科 |
５年 樗木仁美、木村綾花、樋川美優、三上佳奈子、守屋 綺、赤城佳奈、菅原万柚、鈴木玲那、赤羽孝之、熊切 望、渡邊利紀、安部大志、海和 颯、永沼美樹 |
生命薬学科 |
４年 熊谷莉歩、山口和究 |
生命薬学科 |
３年 蛯名成輝、豊嶋翔子 |
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研究の概要
当教室は、長年一貫して病原細菌や真菌の研究を展開してきた。現在、細菌や真菌による感染症の全容を解明
するため、発症に関与する菌側の各種病原因子と宿主の感染防御能についての研究を行っている。この研究を基
礎にして、感染症の新たな診断法や治療法の開発をめざしている。
1) 真菌の病原因子と真菌症
真菌症は、日和見感染症の中で最も重要なものの1つである。我々は、病原性Candida 属酵母の病原性や抗原性
の研究で成果をあげてきた。現在さらに、真菌症の全容解明をめざして、さまざまな環境条件下で病原真菌
（Candida, Malassezia, Aspergillus など）が産生する毒素や酵素の構造と性質の解析を行っている。この
基礎研究から、真菌の多様性を見出すことができたので、迅速で精度の高い診断法やより良い治療法の開発
を検討している。
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2016
1. KRE5 Suppression Induces Cell Wall Stress and Alternative ER Stress Response Required for Maintaining Cell Wall Integrity in
Candida glabrata
Yutaka Tanaka, Masato Sasaki, Fumie Ito, Toshio Aoyama, Michiyo Sato-Okamoto, Azusa Takahashi-Nakaguchi, Hiroji Chibana,
Nobuyuki Shibata
PLoS One., 11, e0161371 (2016).
2. Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance,
and tumor suppression
Takeshi Ueda, Yuichiro Nakata, Akiko Nagamachi, Norimasa Yamasaki, Akinori Kanai, Yasuyuki Sera, Masato Sasaki, Hirotaka Matsui,
Zen-ichiro Honda, Hideaki Oda, Linda Wolff, Toshiya Inaba, Hiroaki, Honda
Proc. Natl. Acad. Sci. U.S.A., 113, 10370-10375 (2016).
3.IDH変異によるエピジェネティック変化と細胞形質
佐々木雅人
生化学, 88, 1-9 (2016).
2015
1. Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus
grown under different culture conditions
Kudoh A., Okawa Y., Shibata N.
Glycobiology, 25, 74-87 (2015).
2. Idh1 protects murine hepatocytes from endotoxin-induced oxidative stress by regulating the intracellular NADP+/NADPH ratio
Itsumi M., Inoue S., Elia A.J., Murakami K., Sasaki M., Lind E.F., Brenner D., Harris I.S., Chio I.I.C., Afzal S., Cairns R.A., Cescon D.W.,
Elford A.R., Ye J., Lang P.A., Li W.Y., Wakeham A., Duncan G.S., Haight J., You-Ten A., Snow B., Yamamoto K., Ohashi P.S., Mak T.W.
Cell Death and Differentiation, 22, 1837-1845 (2015).
3. Mutant IDH is sufficient to initiate enchondromatosis in mice
Hirata M., Sasaki M., Cairns R.A., Inoue S., Puviindran V., Li W.Y., Snow B.E., Jones L.D., Wei Q., Sato S., Tang Y.J., Nadesan P.,
Rokel J., Whetstone H., Poon R., Weng A., Gross S., Straley K., Gliser C., Xu Y., Wunder J., Mak T.W., Alman B.A.
Proc. Natl. Acad. Sci. U.S.A., 112, 2829-2834 (2015).
4. Glutathione and thioredoxin antioxidant pathways synergize to drive cancer initiation and progression
Harris I.S., Treloar A.E., Inoue S., Sasaki M., Gorrini C., Lee K.C., Yung K.Y., Brenner D., Knobbe-Thomsen C.B., Cox M.A.,
Elia A.,Berger T., Cercon D.W., Adeoye A., Brustle A., Molyneux S.D., Mason J.M., Li W.Y., Yamamoto K., Wakeham A., Berman H.K.,
Khokha R., Done S., Kavanagh T.J., Lam C.W., Mak T.W.
Cancer Cell, 27, 211-222 (2015).
2014
1. Largen: a molecular regulator of mammalian cell size control
Kazuo Yamamoto, Valentina Gandin, Masato Sasaki, Susan McCracken, Wanda Li, Jennifer Liepa Silvester, Andrew J. Elia, Feng Wang,
Yosuke Wakutani, Roumiana Alexandrova, Yather D Oo, Peter J. Mullen, Satoshi Inoue, Momoe Itsumi, Valentina Lapin, Jillian Haight,
Andrew Wakeham, Arda Shahinian, Mitsuhiko Ikura, Ivan Topisirovic, Nahum Sonenberg, Tak W. Mak
Mol. Cell, 53, 904-915 (2014).
2. TAp73 is required for spermatogenesis and the maintenance of male fertility
Satoshi Inoue, Richard Tomasini, Alessandro Rufini, Andrew J. Elia, Massimiliano Agostini, Ivano Amelio, Dave Cescon, David Dinsdale,
Lily Zhou, Isaac S. Harris, Sophie Lac, Jennifer Silvester, Wanda Y. Li, Masato Sasaki, Jillian Haight, Anne Brustle, Andrew Wakeham,
Colin Mckerlie, Andrea Jurisicova, Gerry Melino, Tak W. Mak
Proc. Natl. Acad. Sci. U.S.A., 111, 1843-1848 (2014).
3. PTPN12 promotes resistance to oxidative stress and supports tumorigenesis by regulating FOXO signaling
Harris I. S., Blaser H., Moreno J., Treloar A. E., Gorrini C., Sasaki M., Mason J. M., Knobbe C. B., Rufini A., Halle M., Elia A. J.,
Wakeham A., Tremblay M. L., Melino G., Done S., Mak T. W.
Oncogene, 33, 1047-1054 (2014).
2013
1. Identification of Distinct Ligands for the C-type Lectin Receptors Mincle and Dectin-2 in the Pathogenic Fungus Malassezia
Tetsuaki ishikawa, Fumie Itoh, Sayumi Yoshida, Shinobu Saijo, Tetsuhiro Matsuzawa, Tohru Gonoi, Takashi Saito, Yoshio Okawa,
Nobuyuki Shibata, Tomofumi Miyamoto and Sho Yamasaki
Cell Host & Microbe, 13, 477-488 (2013).
2012
1. Difference in fine specificity to polysaccharides of C. albicans mannoprotein between mouse SIGNR1 and human DC-SIGN
Takahara K, Arita T, Tokieda S, Shibata N, Okawa Y, Tateno H, Hirabayashi J, Inaba K.
Infect. Immun., 80, 1699-1706 (2012).
2. Significant differences in the cell wall mannans from three Candida glabrata strains correlate with antifungal drug sensitivity
Shizuka Takahashi, Atsushi Kudoh, Yoshio Okawa, Nobuyuki Shibata
FEBS J., 279, 1844-1856 (2012).
3. Immunochemistry of pathogenic yeast, Candida species, focusing on mannan
Nobuyuki Shibata, Hidemitsu Kobayashi, Shigeo Suzuki
Proc. Jpn. Acad. Ser. B., (2012) in press
2011
1. Chemical structure of β-galactofuranose-containing polysaccharide and O-linked oligosaccharides obtained from the cell wall of pathogenic dematiaceous fungus Fonsecaea pedrosoi
Shibata N., Okawa Y.
Glycobiology, 21, 69-81 (2011).
2. Candida属真菌の細胞壁マンナンの構造と病原性への関与
柴田 信之
東北薬科大学研究誌, 58, 13-27 (2011).
2010
1. Enzymatic synthesis of new oligosaccharides using mannosyltransferases from Candida species and their NMR assignments
Shibata N., Okawa Y.
Biol. Pharm. Bull., 33, 895-899 (2010).
2. Conformational Analysis of β-1,2-Linked Mannobiose to Mannoheptaose, Specific Antigen of Pathogenic Yeast Candida albicans
Shibata N., Okawa Y.
Chem. Pharm. Bull., 58, 1386-1390 (2010).
3.Candida albicans マンナンによるアストロサイトからの炎症性因子の産生
山本 学, 柴田 信之, 大川 喜男
東北薬科大学研究誌, 57, 55-59 (2010).
2009
1. The cell wall galactomannan antigen from Malassezia furfur and Malassezia pachydermatis
contains β-1,6-linked linear galactofuranosyl residues
Shibata N., Saitoh T., Tadokoro Y., Okawa Y.
Microbiology, 155, 3420-3429 (2009).
2. Influence of oxidative and osmotic stresses on the structure of the cell wall mannan of Candida albicans serotype A
Koyama T., Makita M., Shibata N., Okawa Y.
Carbohydr. Res., 344, 2195-2200 (2009).
3. Some properties of β-1,2-mannosyltransferases related to the biosynthesis of the acid-labile oligomannosyl side chains in Candida albicans NIH B-792 strain cells
Goto K., Suzuki A., Shibata N., Okawa Y.
Biol. Pharm. Bull., 32, 1921-1923 (2009).
4. 細胞ELISAを用いたCandida 属酵母表層抗原の検出
大川 喜男, 山田 康代
東北薬科大学研究誌, 56, 47-53 (2009).
2008
1. Primary structure and function of a cytotoxic outer-membrane protein (ComP) of Plesiomonas shigelloides
Tsugawa H., Ogawa A., Takehara S., Kimura M., Okawa Y.,
FEMS Microbiol Lett., 281, 10-16 (2008).
2. Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes
Oyamada H., Ogawa Y., Shibata N., Okawa Y., Suzuki S., Kobayashi H.
Arch. Microbiol., 189, 483-490 (2008).
3. Activity and stability of α-and β-mannosyltransferases in Candida albicans cells cultured at high temperature and at
low pH
Goto K., Okawa Y.,
Biol. Pharm. Bull., 31, 1333-1336 (2008).
4. Comparison of pathogenicity of various Candida tropicalis strains
Okawa Y., Miyauchi M., Kobayashi H.,
Biol. Pharm. Bull., 31, 1507-1510 (2008).
5. 細胞壁マンナン中にβ-1,2-結合をもつ Saccharomycetaceae に属する酵母株の検出
大川 喜男, 山田 康代
東北薬科大学研究誌, 55, 63-67 (2008).
2007
1. Cell adherence-promoted activity of Plesiomonas shigelloides groEL.
Tsugawa H., Ito H., Ohshima M., Okawa Y.,
J. Med. Microbiol., 56, 23-29 (2007).
2. Adhesion of Candida albicans to HeLa cells: Studies using polystyrene beads.
Miyauchi M., Giummelly P., Yazawa S., Okawa Y.,
Biol. Pharm. Bull., 30, 588-590 (2007).
3. Chemical structure of the cell wall mannan of Candida albicans serotype A and its difference in yeast
and hyphal forms.
Shibata N., Suzuki A., Kobayashi H., Okawa Y.,
Biochem. J., 404, 365-372 (2007).
4. Comparison of pathogenicity of various Candida albicans and C. stellatoidea strains.
Okawa Y., Miyauchi M., Takahashi S., Kobayashi H.,
Biol. Pharm. Bull., 30, 1870-1873 (2007).
5.担がんマウスの Candida albicans 感染防御におけるリゾチームの役割
大川 喜男, 小林 真紀子, 鈴木 益子
東北薬科大学研究誌, 54, 69-73 (2007).
2006
1. Disappearance of antigenic factor 6 in Candida glabrata IFO 0622 strain cells cultured at high
temperature.
Okawa Y., Goto K.,
Biol. Pharm. Bull., 29, 187-189 (2006).
2. Structural changes of cell wall mannans of Candida guilliermondii IFO 10279 strain cells cultured at
high temperature.
Okawa Y, Oikawa S., Suzuki S.,
Biol. Pharm. Bull., 29, 388-391 (2006).
3. Isolation, characterization and molecular cloning of a lipolytic enzyme secreted from Malassezia
pachydermatis.
Shibata N, Okanuma N, Hirai K, Arikawa K, Kimura M., Okawa Y
FEMS Microbiol. Lett., 256, 137-144 (2006).
4. Antigenicity of Candida tropicalis strain cells cultured at 27 and 37 degrees C.
Okawa Y., Goto K.,
FEMS Immunol. Med. Microbiol., 46, 438-443 (2006).
5. 真菌細胞壁多糖の構造
柴田 信之, 大川 喜男
日本医真菌学会雑誌, 47, 179-184 (2006).
6. Antigenicity of cell wall mannans of Candida albicans and Candida stellatoidea cultured at high
temperatures in BACTEC medium.
Okawa Y., Goto K.,
Biol. Pharm. Bull., 29, 1723-1727 (2006).
7. Candida albicans マンナンのマクロファージ傷害作用
大川 喜男, 小島 美紀, 石橋 敏広, 中村 佳央, 松田 祐子, 亘理 陽子, 内藤 周一
東北薬科大学研究誌, 53, 57-61 (2006).
2005
1. Antigenicity of cell wall mannans of Candida albicans NIH A-207 strain cells cultured in galactose-
added yeast nitrogen base medium.
Okawa Y., Miyauchi M., Goto K., Giummelly P.,
Biol. Pharm. Bull., 28, 391-393 (2005).
2. Similarity of acid proteinases secreted by Candida albicans NIH A-207 and NIH B-792 strains
cultured in BSA-supplemented medium.
Okawa Y., Suzuki A., Chiba T.,
Biol. Pharm. Bull., 28, 1281-1285 (2005).
3. Invasive phenotype and apoptosis induction of Plesiomonas shigelloides P-1 strain to Caco-2 cells.
Tsugawa H., Ono T., Murakami H., Okawa Y.,
J. Appl. Microbiol., 99, 1435-1443 (2005).
4. 病原性Candida 属酵母の酸性プロテアーゼ産生能の比較
大川 喜男, 小島 芳香, 川嶋 美江子, 岡本 英治
東北薬科大学研究誌, 52, 73-77 (2005).
5. マクロファージの一酸化窒素(NO)産生に及ぼす酵母マンナンの影響
大川 喜男, 藤原 知, 関 史行, 川俣 友紀, 白男川 賢治, 加藤 幸江
東北薬科大学研究誌, 52, 79-83 (2005).
2004
1. Role of polymorphonuclear leukocytes in the resistance of tumor-bearing mice against Candida
albicans infection.
Okawa Y., Kobayashi M., Sakai K., Suzuki M.,
Biol. Pharm. Bull., 27, 674-678 (2004).
2. Isolation and characterization of a cytotoxin produced by Plesiomonas shigelloides P-1 strain.
Okawa Y., Ohtomo Y., Tsugawa H., Matsuda Y., Kobayashi H., Tsukamoto T.,
FEMS Microbiol. Lett., 239, 125-130 (2004).
3.マウス緑膿菌感染に対する酵母マンナンの防御効果
大川 喜男, 小林 眞紀子, 吉田 隆之, 鈴木 益子
東北薬科大学研究誌, 51, 85-89 (2004).
2003
1. Comparative study of protective effects of chitin, chitosan, and N-acetyl chitohexaose against
Pseudomonas aeruginosa and Listeria monocytogenes infections in mice.
Okawa Y., Kobayashi M., Suzuki S., Suzuki M.,
Biol. Pharm. Bull., 26, 902-904 (2003).
2. A new mannoheptaose containing alpha and β-1,2-linkages isolated from the mannan of Torulaspora
delbrueckii: ELISA inhibition studies.
Okawa Y., Monma K., Shibata N., Kobayashi H., Yamada Y.,
Carbohydr. Res., 338, 1175-1182 (2003).
3. Existence of novel β-1,2-linkage-containing side chain in the mannan of Candida lusitaniae,
antigenically related to Candida albicans serotype A.
Shibata N., Kobayashi H., Okawa Y., Suzuki S.,
Eur. J. Biochem., 270, 2565-2575 (2003).
4. マウスListeria monocytogenes 感染に対する酵母マンナンの防御機構について
大川 喜男, 小林 眞紀子, 鈴木 益子
東北薬科大学研究誌, 50, 149-154 (2003).
2002
1. Delayed lethal response to Aspergillus fumigatus infection in sarcoma 180 tumor-bearing mice.
Okawa Y., Murata Y., Suzuki M., Ito M., Hotchi M., Suzuki S.,
FEMS Immunol. Med. Microbiol., 34, 113-117 (2002).
2. A novel protein with alkaline phosphatase and protease inhibitor activities in Streptomyces
hiroshimensis.
Nitta M., Goto M., Shibuya N., Okawa Y.,
Biol. Pharm. Bull., 25, 833-836 (2002).
3. Lethality of yeasts with low pathogenicity in mice immunocompromised by cyclophosphamide
treatment.
Okawa Y., Yamada Y.,
Biol. Pharm. Bull., 25, 940-942 (2002).
4. Production of interleukin-1 activity of kupffer cells from mice treated with the acidic mannan fraction
of baker's yeast.
Okawa Y., Abe K., Watanabe T., Sasaki H., Suzuki M.,
Biol. Pharm. Bull., 25, 1506-1508 (2002).
5. 酵母マンナン投与マウス肝クッパー細胞によるCandida albicans の殺菌
大川 喜男, 阿部 公二, 渡部 俊彦, 佐々木 弘好, 鈴木 益子
東北薬科大学研究誌, 49, 103-107 (2002).
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TOHOKU PHARMACEUTICAL UNIVERSITY All Rights Reserved |
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