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01 Organic and Medicinal Chemistry

Research Projects

  • Ohta, K.; Goto, T.; Fujii, S.; Kawahata, M.; Oda, A.; Ohta, S.; Yamaguchi, K.; Hirono, S.; Endo, Y.
    Crystal Structure, Docking Study and Structure-activity Relationship of Carborane-containing Androgen Receptor Antagonist 3-(12-Hydroxymethyl-1,12-dicarba-closo-dodecaboran-1-yl)- benzonitrile.
    Bioorg. Med. Chem., 2011,19, 3540-3548.
  • Songkram, C.; Ohta, K.; Yamaguchi, K.; Pichierri, F.; Endo, Y.
    Conformational Control of Benzyl-o-carboranylbenzene Derivatives and Molecular Encapsulation of Acetone into the Dynamically-formed Space of 1,3,5-Tris(2-benzyl-o- carboran-1-yl)benzene.
    Inorg. Chem., 2010, 49, 11174-11183.
  • Goto, T.; Ohta, K., Fujii, S.; Ohta, S.; Endo, Y.
    Design and Synthesis of Androgen Receptor Full Antagonists Bearing a p-Carborane Cage: Promising Ligands for Anti-androgen Withdrawal Syndrome.
    J. Med. Chem., 2010, 53, 4917-4926.
  • Ohta, K.; Ogawa, T.; Suzuki, T.; Ohta, S.; Endo. Y. Novel Estrogen Receptor (ER) Modulators: Carbamate and Thiocarbamate Derivatives with m-Carborane Bisphenol Structure. Bioorg. Med. Chem., 2009, 17, 7958-7963.
  • Ohta, K.; Yamazaki, H.; Endo, Y.
    Magnesium-Assisted Intramolecular Demethylation Utilizing Carborane C-H Geometry
    J. Organomet. Chem., 2009, 694, 1646-1651.
  • Ogawa, T.; Ohta, K.; Iijima, T.; Suzuki, T.; Ohta, S.; Endo, Y.
    Synthesis and Biological Evaluation of p-Carborane Bisphenols and Their Derivatives: Structure-activity Relationship for Estrogenic Activity
    Bioorg. Med. Chem., 2009, 17, 1109-1117.
  • Ohta, K.; Konno, S; Endo, Y.
    Complexation of -Cyclodextrin with Carborane Derivatives in Aqueous Solution
    Tetrahedron Lett. 2008, 49, 6525-6528.
  • Ohta, K.; Chiba, Y.; Ogawa, T.; Endo, Y.
    Promising Core Structure for Nuclear Receptor Ligands: Design and Synthesis of Novel Estrogen Receptor Ligands Based on Diphenylamine Skeleton
    Bioorg. Med. Chem. Lett., 2008, 18, 5050-5053.
  • Ohta, K.; Yamazaki, H.; Pichierri , F.; Kawahata, M.; Yamaguchi, K.; Endo, Y.
    Solid-state Supramolecular Array through Cooperative – Interactions of 1-(2-Methoxyphenyl)-o-carborane
    Tetrahedron, 2007, 63, 12160-12165.
  • Ohta, K.; Yamazaki, H.; Kawahata, M.; Yamaguchi, K.; Pichierri, F.; Endo, Y.
    Proton-Driven Conformational Change in a 2-Aryl-p-carborane Constrained by an Intramolecular C-H—O Hydrogen Bond
    Tetrahedron. Lett., 2007, 48, 5231-5234.
  • Ohta, K.; Goto, T.; Yamazaki, H.; Pichierri, F.: Endo, Y.
    Facile and Efficient Synthesis of C-Hydroxycarboranes and C,C’-Dihydroxycarboranes
    Inorg. Chem., 2007, 46, 3966-3970.
  • Nagamine, T.; Inomata, K.; Endo, Y.; Paquette, L. A.
    Amino Acid-mediated Intramolecular Asymmetric Aldol Reaction to Construct a New Chiral Bicyclic Enedione Containing a 7-Membered Ring: Remarkable Inversion of Enantioselectivity Compared to the 6-Membered Ring.
    J. Org. Chem., 2007, 72, 123-131.
  • Ohta, K.; Goto, T.; Endo, Y.
    1,2-Dicarba-closo-dodecaboran-1-yl Naphthalene Derivatives.
    Inorg. Chem., 2005, 23, 8569-8573.
  • Goto, T.; Ohta, K.; Suzuki, T.; Ohta, S.; Endo, Y.
    Design and Synthesis of Novel Androgen Receptor (AR) Antagonists with Sterically Bulky Icosahedral Carboranes.
    Bioorg. Med. Chem., 2005, 13, 6414-6424.
  • Fujii, S.; Goto, T.; Ohta, K.; Hashimoto, Y.; Suzuki, T.; Ohta, S.; Endo, Y.
    Potent Androgen Antagonists Based on Carborane As a Hydrophobic Core Structure.
    J. Med. Chem. 2005, 48, 4654-4662.
  • Endo, Y.; Yoshimi, T.; Ohta, K.; Suzuki, T.; Ohta, S.
    Potent Estrogen Receptor Ligands Based on Bisphenols with Globular Hydrophobic Cores.
    J. Med. Chem. 2005, 48, 3941-3944.
  • Endo, Y.; Songkram, C.; Ohta, K.; Kaszynski, P.; Yamaguchi, K.
    A Distorted Benzene Bearing Two Bulky Substituents on Adjacent Positions: Structure of 1,2-Bis(1,2-Dicarba-closo-dodecaboran-1-yl)benzene.
    Tetrahedron Lett., 2005, 46, 699-702.
  • Ohta, K.; Iijima, T.; Kawachi, E.; Kagechika, H.; Endo, Y
    Novel Retinoid X Receptor (RXR) Antagonists Having a Dicarba-closo-dodecaborane as a Hydrophobic Moiety.
    Bioorg. Med. Chem. Lett. 2004, 14, 5913-5918.
  • Suzuki, K.; Inomata, K.; Endo, Y.
    Enantiocontrllooed Synthesis of Jasmonates via Tandem Retro-Diels-Alder-Ene reaction Activated by a Silyl Substituent
    Org. Lett., 2004, 6, 409-411.
  • Endo, Y.; Yamamoto, K.; Kagechika, H.
    Utility of Boron Clusters for Drug Design. Relation between Estrogen Receptor Binding Affinity and Hydrophobicity of Phenols Bearing Various Types of Carboranyl Groups.
    Bioorg. Med. Chem. Lett. 2003, 13, 4089-4092.

02 Organic and Pharmaceutical Chemistry

Research Projects

  • Yoshimura, Y.; Yamazaki, Y.; Saito, Y.; Natori, Y.; Imamichi, T.; Takahata, H. “Synthesis of 5-Thiodidehydropyranylcytosine Derivatives as Potential anti-HIV Agents”
    Bioorg. Med. Chem. Lett. 2011, 21, 3313-3316.
  • Natori, Y.; Imahori, T.; Murakami, K.; Yoshimura, Y.; Nakagawa, S.; Kato, A.; Adachi, I.; Takahata, H. “The synthesis and biological evaluation of 1-C-alkyl-L-arabinoiminofuranoses, a novel class of -glucosidase inhibitors”
    Bioorg. Med. Chem. Lett. 2011, 21, 738-741.
  • Saito, Y.; Yoshimura, Y.; Takahata, H. “Chemoselective O-tert-butoxycarbonylation of phenols using 6,7-dimethoxyisoquinoline as a novel organocatalyst” Tetrahedron Lett. 2010, 51, 6915-6917.
  • Yoshimura, Y.; Asami, K.; Imamichi, T.; Kuroda, T.; Shiraki, K.; Takahata, H. “Design and Synthesis of Isonucleosides Constructed on a 2-Oxa-6-thiabicyclo[3.2.0]heptane Scaffold”
    J. Org. Chem. 2010, 75, 4161-4171.
  • Yoshimura, Y.; Yamazaki, Y.; Saito, Y.; Takahata, H. ”Synthesis of 1-(5,6-Dihydro-2H-thiopyran-2-yl)uracil by a Pummerer-type Thioglycosylation Reaction: The Regioselectivity of Allylic Substitution”
    Tetrahedron 2009, 65, 9091-9102.
  • Saito, Y.; Ouchi, H.; Takahata, H. “Carboxamidation of carboxylic acids with 1-tert-butoxy-2-tert-butoxycarbonyl-1,2-dihydroisoquinoline (BBDI) without base”
    Tetrahedron 2008, 64, 11129-11135
  • Imahori, T.; Ojima, H.; Yoshimura, Y.; Takahata, H. “Acceleration effect of allylic hydroxy group on ring-closing enyne metathesis of terminal alkynes: scope, application and mechanistic insights”
    Chem. Eur. J. 2008, 14, 10762-10771.
  • Yoshimura, Y.; Ohta, M.; Imahori, T.; Imamichi, T.; Takahata, H. “A New Entry to Carbocyclic Nucleosides: Oxidative Coupling Reaction of Cycloalkenylsilanes with a Nucleobase Mediated by Hypervalent Iodine Reagent”
    Org. Lett. 2008, 10, 3449-3452
  • Ohara, C.; Takahashi, R.; Miyagawa, T.; Yoshimura, Y.; Kato A.; Adachi, I.; Takahata, H. Synthesis of all stereoisomers of 3-hydroxypipecolic acid and 3-hydroxy-4,5-dehydropipecolic acid and their evaluation as glycosidase inhibitors
    Bioorg. Med. Chem. Lett. 2008, 18, 1810-1813.
  • Saito, Y.; Shinkai, T.; Yoshimura, Y.; Takahata, H. “A straightforward stereoselective synthesis of meso-(S,S)-and (R,R)-2,6-diaminopimelic acids from cis-1,4-diacetoxycyclohept-2-ene”
    Bioorg. Med. Chem. Lett. 2007, 17, 5894-5896.
  • Mihara, Y.; Ojima, H.; Imahori, T.; Yoshimura, Y.; Ouchi, H.; Takahata, H. “Asymmetric Synthesis of All Stereoisomers of Isofagomine Using [2,3]-Wittig Rearrangement”
    Heterocycles 2007, 72, 633-645.
  • Takahata, H.; Suto, Y.; Kato, E.; Yoshimura, Y.; Ouchi, H. “A New Preparation of Homochiral N-Protected 5-Hydroxy-3-piperidenes, Promising Chiral Building Blocks, by Palladium-Catalyzed Deracemization of Their Alkyl Carbonates”
    Adv. Syn. & Cat. 2007, 349, 685-693.
  • Yoshimura, Y.; Asami, K.; Matsui, H.; Tanaka, H.; Takahata, H. “New Stnthesis of (±)-Isonucleosides”
    Org. Lett. 2006, 8, 6015-6018.
  • Saito, Y.; Ouchi, H.; Takahata, H. “A Novel tert-butoxycarbonylation reagent: 1-tert-Butoxy-2-tert-butoxycarbonyl-1,2-dihydroisoquinoline (BBDI) ”
    Tetrahedron 2006, 62, 11599-11607.
  • Saito, Y.; Watanabe, T.; Takahata, H. “A novel 1-tert-butoxy-2-tert-butoxycarbonyl-1,2-dihydroisoquinoline (BBDI)-catalyzed esterification of N-protected amino acids with nearly equimolar amounts of alcohols in the presence of Boc2O”
    Tetrahedron Lett. 2006, 47, 3099-3102.
  • Ouchi, H.; Mihara, Y.; Takahata, H. “A New Route to Diverse 1-Azasugars from N-Boc-5-hydroxy-3-piperidene as Common Building Block”
    J. Org. Chem. 2005, 70, 5207-5214.
  • Kato, A.; Kato, N.; Kano, E.; Adachi, I.; Ikeda, K.; Yu, L.; Okamoto, T.; Banba, Y.; Ouchi, H.; Takahata, H.; Asano, N. “Biological Properties of D- and L-1-Deoxyazasugars”
    J. Med. Chem. 2005, 48, 2036-2044.
  • Takahata, H.; Banba, Y.; Ouchi, H.; Nemoto, H. “Concise and Highly Stereocontrolled Synthesis of 1-Deoxygalactonojirimycin and Its Congeners Using Dioxanylpiperidene, a Promising Chiral Building Block”
    Org. Lett. 2003, 5, 2527-2529.
  • Takahata, H.; Ouchi, H.; Ichinose, M.; Nemoto, H. “A Novel C2-Symmetric 2,6-Diallylpiperidine Carboxylic Acid Methyl Ester as a Promising Chiral Building Block for Piperidine-related Alkaloids”
    Org. Lett. 2002, 4, 3459-3462.
  • Ouchi, H.; Saito, Y.; Yamamoto, Y.; Takahata, H. “1-tert-Butoxy-2-tert-butoxycarbonyl-1,2-dihydroisoquinoline: A Novel and Chemoselective tert-Butoxycarbonylation Reagent”
    Org. Lett. 2002, 4, 585-587.

03 Clinical Infectious Diseases and Chemotherapy

Research Projects

  • Fuse K, Fujimura S, Kikuchi T, Gomi K, Iida Y, Nukiwa T, Watanabe A Reduction of virulence factor pyocyanin production in multidrug resistant Pseudomonas aeruginosa J Infect Chemother 19: 82-88, 2013
  • Takahashi H, Fujimura S, Ubukata S, Sato E, Shoji M, Utagawa M, Kikuchi T, Watanabe A. Pneumonia after earthquake, Japan, 2011 Emerg Infect Dis 18: 1909-1911, 2012
  • Fujimura S, Watanabe A. Generic antibiotics in Japan. J Infect Chemother 18: 421-427, 2012
  • Fujimura S, Watanabe A, Kimura K, Kaji M. Probiotic mechanism of Lactobacillus gasseriOLL2716 strain against Helicobacter pylori. J Clin Microbiol. 50: 1134-1136, 2012
  • Daito H, Kikuchi T, Sakakibara T, Gomi K, Damayanti T, Zaini J, Tode N, Kanehira M, Koyama S, Fujimura S, Ebina M, Ishii KJ, Akira S, Takai T, Watanabe A, Nukiwa T.  Mycobacterial hypersensitivity pneumonitis requires TLR9-MyD88 in lung CD11b+CD11c+ cells. Eur Respir J 38: 688-701, 2011
  • Gomi K, Fujimura S, Fuse K, Takane H, Nakano Y, Kariya Y, Kikuchi T, Kurokawa I, Tokue Y, Watanabe A. Antibacterial activity of carbapanems against clinical isolates of respiratory bacterial pathogens in the Northeastern region of Japan in 2007. J Infect Chemother 17: 200-206, 2011
  • Fujimura S, Fuse K, Takane H, Nakano Y, Gomi K, Kikuchi T, Watanabe A. Antibacterial effects of brand-name teicoplanin and generic products against clinical isolates of methicillin-resistant Staphylococcus aureus. J Infect Chemother 17: 30-33, 2011
  • Fujimura S, Nakano Y, Takane H, Kikuchi T, Watanabe A. Risk factors for healthcare-associated pneumonia: Transmission of multidrug-resistant Pseudomonas aeruginosa isolates from general hospitals to nursing homes. Am J Infect Control 39: 173-175, 2011
  • Fujimura S, Watanabe A. National survey on antimicrobial therapy compliance in Japan. Scand J Infect Dis 42: 637-638, 2010
  • Kato S, Fujimura S. Primary antimicrobial resistance of Helicobacter pylori in children during the past 9 years. Pediatric Int 52: 187-90, 2010
  • Fujimura S, Takane H, Nakano Y, Watanabe A. In vitro synergy studies based on tazobactam/piperacillin against clinical isolates of metallo-beta-lactamase-producing Pseudomonas aeruginosa. J Antimicrob Chemother 64:1115-1116, 2009
  • Kikuchi T, Watanabe A, Gomi K, Sakakibara T, Nishimori K, Daito H, Fujimura S, Tazawa R, Inoue A, Ebina M, Tokue Y, Kaku M, Nukiwa T. Association between mycobacterial genotypes and disease progression in Mycobacterium avium pulmonary infection. Thorax 64:901-907, 2009
  • Fujimura S, Sato T, Kikuchi T, Zaini J, Gomi K, Watanabe A. Efficacy of clarithromycin plus vancomycin in mice with implant-related infection caused by biofilms-forming Staphylococcus aureus. J Orthoped Sci 14: 658-661, 2009
  • Fujimura S, Kato S, Watanabe A. Water source as a Helicobacter pylori transmission route: a 3-year follow-up study of Japanese children living in a unique district. J. Med. Microbiol. 57:909-910, 2008
  • Fujimura S, Sato T, Mikami T, Kikuchi T, Gomi K and Watanabe A: Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titanium medical divices. Intern J Antimicrob Agents 32:481-484, 2008.
  • Fujimura S, Fuse K, Gomi K, Kikuchi T, Tokue Y and Watanabe A: In vitro susceptibility of clinical isolates of methicillin-resistant Staphylococcus aureus to manufactured generic drugs compared with the brand vancomycin. Intern J Antimicrob Agents 31:391-392, 2008
  • Watanabe A, Fujimura S, Kikuchi T, Gomi K, Fuse K and Nukiwa T: Evaluation of dosings of carbapenems for severe respiratory infection using Monte Carlo simulation. J Infect Chemother 13: 332-340, 2007
  • Fujimura S, Nakano Y, Sato T, Shirahata K and Watanabe A: Relationship between the usage of carbapenem antibiotics and the incidence of imipenem-resistant Pseudomonas aeruginosa. J Infect Chemother 13:147-150, 2007
  • Gomi K, Watanabe A, Aoki S, Kikuchi T, Fuse K, Nukiwa T, Kurokawa I and Fujimura S: Antibacterial activity of carbapenems against clinically isolated respiratory bacterial pathogens in Japan between 2005 and 2006. Intern J Antimicrob Agents 29:586-592, 2007
  • Fujimura S, Oka T, Tooi O, Meguro M, Chiba M, Kawamura M, Maki F, Takeda H, Watanabe A. Detection of L. pneumophila serogroup 7 strain from bathwater samples in a Japanese hospital. J Infect Chemother 12:105-108, 2006
  • Fujimura S, Kato S, Oda M, Miyahara M, Ito Y, Kimura K, Kawamura T, Ohnuma M, Tateno H, Watanabe A. Detection of L.gasseri OLL2716 strains administrated with Yogurt drink in gastric mucus layer in human. Lett Appl Microbiol 43:578-581, 2006
  • Gomi K, Kikuchi T, Tokue Y, Fujimura S, Uehara A, Takada H, Watanabe A and Nukiwa T: Mouse and human cells activation by N-dodecanoyl-DL-homoserine lactone(C12-HSL), aChromobacterium violaceum autoinducer. Infect Immun, 74:7029-7031, 2006
  • Kato S, Fujimura S, Oda M, Kimura K, Hamada M Non-Helicobacter bacterial flora rarely develops in the gastric mucosal layer of children. Dig Dis Sci 51:641-646 2006

04 Synthetic and Medicinal Chemistry

Research Projects

  • Sakurai, J.; Kikuchi, T.; Takahashi, O.; Watanabe, K.; Katoh, T.; “Enantioselective total synthesis of (+)-stachyflin: A potential anti-influenza A virus agent isolated from a microorganism”
    Eur. J. Org. Chem. 2011, 16, 2948-2957.
  • Watanabe, K.; Sakurai, J.; Abe, H.; Katoh, T.; ” Total synthesis of (+)-stachyflin: a potential anti-influenza A virus agent”
    Chem. Commun. 2010, 46, 4055-4057.
  • Oguchi, T.; Watanabe, K.; Ohkubo, K.; Abe, H.; Katoh, T.; ” Enantioselective total synthesis of (-)-candelalides A, B and C: Potential Kv1.3 blocking immunosuppressive agents”
    Chem. Eur. J. 2009, 15, 2826-2845.
  • Narita, K.; Kikuchi, T.; Watanabe, K.; Takizawa, T.; Oguchi, T.; Kudo, K.; Matsuhara, K.; Abe, H.; Yamori, T.; Yoshida, M.; Katoh T.; “Total synthesis of the bicyclic depsipeptide HDAC inhibitors spiruchostatins A and B, 5”-epi-spiruchostatin B, FK228 (FR901228) and preliminary evaluation of their biological activity”
    Chem. Eur. J. 2009, 15, 11174-11186,
  • Suzuki, T.; Ghozati, K.; Katoh, T.; Sasai, H.; “Ir-catalyzed oxidative desymmetrization of meso-diols”
    Org. Lett. 2009, 11, 4286-4288.
  • Sakurai, J.; Oguchi, T.; Watanabe, K.; Abe, H.; Kanno, S.; Ishikawa, M.; Katoh, T.; “Highly efficient total synthesis of the marine natural products (+)-avarone, (+)-avarol, (-)-neoavarone, (-)-neoavarol and (+)-aureol”
    Chem. Eur. J. 2008, 14, 829-837.
  • Takizawa, T.; Watanabe, K.; Narita, K.; Oguchi, T.; Abe, H.; Katoh, T.; “Total synthesis of spiruchostatin B, a potent histone deacetylase inhibitor, from a microorganism”
    Chem. Commun. 2008, 14, 1677-1679.
  • Araki, H.; Inoue, M.; Suzuki, T.; Yamori, T.; Kohno, M.; Watanabe, K.; Abe, H.; Katoh, T.; “Enantioselective total synthesis of (+)-ottelione A, (-)-ottelione B, (+)-3-epi-ottelione A and preliminary evaluation of their antitumor activity”
    Chem. Eur. J. 2007, 13, 9866-9881.
  • Araki, H.; Katoh, T.; Inoue, M.; “Iterative two-step strategy for C(2)-C(4′) linked poly-oxazole synthesis using Suzuki-Miyaura cross-coupling reaction”
    Tetrahedron Lett. 2007, 48, 3713-3717.
  • Inoue, M.; Yokota, W.; Katoh, T.; “Enantioselective total synthesis of (+)-scyphostatin, a potent and specific inhibitor of neutral sphingomyelinase”
    Synthesis 2007, 622-637.
  • Abe, T.; Iwasaki, K.; Inoue, M.; Suzuki, T.; Watanabe, K.; Katoh, T.; “Convergent and enantioselective total synthesis of (-)-nalanthalide, a potential Kv1.3 blocking immunosuppressant”
    Tetrahedron Lett. 2006, 47, 3251-3255.
  • Katoh, T.; Izuhara, T.; Yokota, W.; Inoue, M.; Watanabe, K.; Nobeyama, A.; Suzuki, T.; “Enantiocontrolled synthesis of the epoxycyclohexenone moieties of scyphostatin, a potent and specific inhibitor of neutral sphingomyelinase” Tetrahedron 2006, 62, 1590-1608.
  • Asano, M.; Inoue, M.; Watanabe, K.; Abe, H.; Katoh, T.; “Synthetic studies toward GKK1032s, novel antibiotic antitumor agents: Enantioselective synthesis of the fully elaborated tricyclic core via an intramolecular Diels-Alder cycloaddition”
    J. Org. Chem. 2006, 71, 6942-6951.
  • Araki, H.; Katoh, T.; Inoue, M.; “Synthesis and reaction of the first oxazol-4-ylboronates: Useful reagents for the preparation of the oxazole-containing biaryl compounds”
    Synlett 2006, 4, 555-558.
  • Watanabe, K.; Iwasaki, K.; Abe, T.; Inoue, M.; Ohkubo, K.; Suzuki, T.; Katoh, T.; “Enantioselective total synthesis of (-)-candelalide A, a novel blocker of the voltage-gated potassium channel Kv1.3 for an immunosuppressive agent”
    Org. Lett. 2005, 7, 3745-3748.
  • Asano, M.; Inoue, M.; Katoh, T.; “Model studies towards the total synthesis of GKK1032s, novel antibiotic anti-tumor agents: Enantioselective synthesis of the alkyl aryl ether portion of GKK1032s”
    Synlett 2005, 17, 2599-2602.
  • Asano, M.; Inoue, M.; Katoh, T.; “Enantioselective synthesis of the tricyclic core of GKK1032, novel antibiotic anti-tumor agents, by employing an intramolecular Diels-Alder cycloaddition strategy”
    Synlett 2005, 10, 1539-1542.
  • Suzuki, T.; Matsuo, T.; Watanabe, K.; Katoh, T.; “Iridium-catalyzed oxidative dimerization of primary alcohols to esters using 2-butanone as an oxidant”
    Synlett 2005, 9, 1453-1455.
  • Suzuki, T.; Yamada, T.; Matsuo, T.; Watanabe, K.; Katoh, T.; “Tishchenko reaction using an iridium-ligand bifunctional catalyst”
    Synlett 2005, 9, 1450-1452.
  • Inoue, M.; Yokota, W.; Murugesh, M. G.; Izuhara, T.; Katoh, T.; “Total synthesis of (+)-scyphostatin, A potent and specific inhibitor of neutral sphingomyelinase”
    Angew. Chem. , Int. Ed. 2004, 43, 4207-4209.

05 Bioanalytical Chemistry

Research Projects

  • Asai, T.; Luo, D.; Yamashita, K.; Oshima, Y. “Structure and biomimetic synthesis of novel a-pyrone polyketides of an endophytic penicillium sp. Catharanthus roses”
    Org. Lett. 2013, 15, 1020-1023.
  • Asai, T.; Otsuki, S.; Sakurai, H.; Yamashita, K.; Ozeki, T.; Oshima, Y. “Benzophenones from an endophytic fungus, Graphiopsis chlorocephala, from Paeonia lactiflora cultivated in the presence of an NAD+-dependent HDAC inhibitor”
    Org. Lett. 2013, 15, 2058-2061.
  • Asai, T.; Otsuki, S.; Taniguchi, T.; Monde, K.; Yamashita, K.; Sakurai, H.; Ozeki, T.; Oshima Y. “Structures and absolute configurations of short-branched fatty acid dimers from an endophytic fungus of Aloe arborescens”
    Tetrahedron Lett. 2013, 54, 3402-3405.
  • Asai, T.; Yamamoto, T.; Chung, Y.-M.; Chang, F.-R.; Wu, Y. M.; Yamashita, K.; Oshima, Y. “Aromatic polyketide glycosides from an entomopathogenic fungus, Cordyceps indigotica”
    Tetrahedron Lett. 2012, 53, 277-280.
  • Asai, T.; Chung, Y.-M.; Sakurai, H.; Ozeki, T.; Chang, F.-R.; Yamashita, K.; Oshima, Y. “Tenuipyrone, a novel skeletal polyketide from the entomopathogenic fungus, Isaria tenuipes, cultivated in the presence of epigenetic modifiers”
    Org. Lett. 2012, 14, 513-515.
  • Ohno, K.; Nakata, C.; Sano, Y.; Nishikawa, F.; Nishikawa, S.; Arakawa, H. “Development of RNA aptamer and its ligand binding assay on microchip electrophoresis”
    Curr. Chem. Genomics 2012, 6, 1-5.
  • Asai, T.; Luo, D.; Obara, Y.; Taniguchi, T.; Monde, K.; Yamashita, K.; Oshima, Y.; “Dihydrobenzofurans as cannabinoid receptor ligands from Cordyceps annullata, an entomopathogenic fungus cultivated in the presence of an HDAC inhibitor”
    Tetrahedron Lett. 2012, 53, 2239-2243.
  • Hashimoto, E. H.; Kamogae, M.; Vanzella, T. P.; Colus, I. M. S.; Bracarense, A. P. F. R. L.; Bitterncourt-Oliveria, M. C.; Itano, E.; Kuroda, E. K.; Katoh, H.; Nagata, S.; Ueno, Y.; Harada, K.; Hirooka, E. Y. “Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays”
    Braz. Arch. Biol. Technol. 2012, 56, 151-159.
  • Asai, T.; Chung, Y.-M.; Sakurai, H.; Ozeki, T.; Chang, F.-R.; Wu, Y.-C.; Yamashita, K.; Oshima, Y. “Highly oxidized ergosterols and isariotin analogs from an entomopathogenic fungus, Gibellula formosana, cultivated in the presence of epigenetic modifying agents”
    Tetrahedron 2012, 68, 5817-5823.
  • Nakanishi, K.; Takagi, M.; Zaitsu, K.; Shima, N.; Kamata, H.; Miki, A.; Kato, H.; Harada, K.; Tsutihashi, H.; Suzuki, K. “Simultaneous enantiomeric determination of MDMA and its phase I and phase II metabolites in urine by liquid chromatography-tandem mass spectrometry with chiral derivatization”
    Anal. Bioanal. Chem. 2012, 404, 2427-2435.
  • Arakawa, H.; Nakabayashi, S.; Ohno, K.; Maeda, M. “New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA”
    J. Pharmaceut. Anal. 2012, 2, 156-159.
  • Nakamura, Y.; Satoh, F.; Morimoto, R.; Kudo, M.; Takase, K.; Gometz-Sanchez, C. E.; Honma, S.; Okuyama, M.; Yamashita, K.; Rainey, W. E.; Sasano, H.; Ito, S. “18-Oxocortisol measurement in adrenal vein sampling as a biomarker for subclassifying primary aldosteroism”
    J. Clin. Endocrinol. Metab. 2011, 96, E1272-1278.
  • Harada, K.; Kato, H.; Tsuji, K. “Antimicrobial activity of various antibiotics against B-9 strain that degrades microcystin produced by cyanobacteria”
    J. Res. Inst. Meijo. Univ. 2011, 10, 35-43.
  • Yamashita, K.; Yamazaki, K.; Komatsu, S.; Numazawa, M. “Fusaric acid as a novel proton-affinitive derivatizing reagent for highly sensitive quantification of hydroxysteroids by LC-ESI-MS/MS”
    J. Am. Soc. Mass Spectrom. 2010, 21, 249-253.
  • Yamashita, K.; Masuda, A.; Hoshino, Y.; Komatsu, S.; Numazawa, M. “Assay of labile estrogen o-quinones, potent carcinogenic molecular species, by high performance liquid chromatography-electrospray ionization tandem mass spectrometry with phenazine derivatization”
    J. Steroid Biochem. Mol. Biol. 2010, 119, 141-148.
  • Takahashi, M.; Yamashita, K.; Numazawa, M. “Probing the binding pocket of the active site of aromatase with 2-phenylaliphatic androsta-1,4-diene-3,17-dione steroids”
    Steroids 2010, 75, 330-337.
  • Takahashi, M.; Yamashita, K.; Numazawa, M. “Probing the binding pocket of the active site of aromatase with 2-phenylaliphatic androsta-1,4-diene-3,17-dione steroids”
    Steroids 2010, 75, 330-337.
  • Honda, A.; Miyazaki, T.; Ikegami, T.; Iwamoto, J.; Yamashita, K.; Numazawa, M.; Matsuzaki Y. “Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS”
    J. Steroid Biochem. Mol. Biol. 2010, 121, 556-564.
  • Watari, Y.; Yamaguchi, S.; Takahashi, M.; Nagaoka, M.; Numazawa, M. “4- and 6-(p-Sulphamoylphenyl)androstenediones: Studies of aromatase inhibitor-based oestrone sulphatase inhibition”
    Steroids 2010, 75, 891-896.
  • Honda, A.; Yamashita, K.; Hara, T.; Ikegami, T.; Miyazaki, T.; Shirai, M.; Xu, G.; Numazawa, M.; Matsuzaki, Y. “Highly sensitive quantification of key regulatory oxysterols in biological samples by LC-ESI-MS/MS”
    J. Lipid Res. 2009, 50, 350-357.
  • Numazawa, M.; Yamashita, K.; Kimura, N.; Takahashi, M. “Chemical aromatization of 19-hydroxyandrosta-1,4-diene-3,17-dione with acid or alkaline: Elimination of the 19-hydroxy methyl group as formaldehyde”
    Steroids 2009, 74, 208-211.

07 Microbiology

Research Projects

  • Watanabe, T.; Irokawa, H.; Ogasawara, A.; Iwai, K.; and Kuge, S. “Requirement of peroxiredoxin on the stationary phase of yeast cell growth” J. Toxicol. Sci. 2014, 39, 51-58.
  • Kubota, N.; Inayoshi, Y.; Satoh, N.: Fukuda, T.; Iwai, K.; Tomoda, H.; Kohara, M.; Kataoka, K.; Shimamoto, A.; Furuichi, Y.; Nomoto, A.; Naganuma, A.; Kuge, S. “HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells” FEBS Lett. 2012, 586, 2318-2325.
  • Iwai,K.; Naganuma,A.; Kuge,S. “Peroxiredoxin Ahp1 acts as a receptor for alkylhydroperoxides to induce disulfide bond formation in the Cad1 transcription factor” J. Biol. Chem. 2010, 285, 10597-10604.
  • Yano,T.; Oku,M.; Akeyama,N.; Itoyama,A.; Yurimoto,H.; Kuge,S.; Fujiki,Y.; Sakai,Y. “A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes” Mol Cell Biol. 2010, 30, 3758-3766.
  • Hwang,G.W.; Hayashi,T.; Kita,K.; Takahashi,T.; Kuge,S.; Naganuma,A. “Overexpression of the novel F-box protein Ymr258c confers resistance to methylmercury in Saccharomyces cerevisiae” J. Toxicol. Sci. 2009, 34, 413-416.
  • Ueno,Y.; Maruyama,N.; Kanno,M.; Watanabe,T.; Ogasawara,A.; Mikami,T.; Matsumoto,T. “Effect of propranolol on hyphae formation signal in Candida albicansBiol. Pharm. Bull. , 2009, 129-131.
  • Tachibana,T.; Okazaki,S.; A. Murayama,A.; Naganuma,A.; Nomoto,S.; Kuge,S. “A major-peroxiredoxin-induced activation of Yap1 transcription factor is mediated by reduction-sensitive disulfide-bonds and reveals a low level of transcriptional activation” J. Biol. Chem.2008, 284, 4464-4472.
  • Fujimura,S.; Sato,T.; Mikami,T.; Kikuchi,T.; Gomi,K.; Watanabe,A. “Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titanium medical devicesInt. J. Antimicrobial Agent. 2008, 32, 481-484.
  • Ogasawara, A.; Iino,Y.; Sato,K.; Nakajima,Y.; Bessho,S.; Watanabe,T.; Mikami,T.; Matsumoto,T. “Anti-Candida Activity of Sodium Sulfite” Biol. Pharm. Bull. , 2008, 31, 1101-1103.
  • Okazaki,S.; Tachibana,T.; Naganuma,A.; Mano,N.; Kuge,S. “Multistep disulfide bond formation in Yap1 is required for sensing and transduction of H2O2 stress signal” Mol. Cell, 2007, 27, 675-688.
  • Nakagawa,S.; Umehara,T.; Matsuda,C.; Kuge,S.; Sudoh,M.; Kohara, M. “Hsp90 inhibitors suppress HCV replication in replicon cells and humanized liver mice” Biochem. Biophys. Res. Commun. , 2007, 353, 882-888.
  • Kubota,N.; Naganuma,A.; Kuge,S. “DNA microarray analysis of transcriptional responses of yeast cells to expression of core protein of hepatitis C virus” J. Toxicol. Sci. , 2007, 32,: 201-204.
  • Hwang,G.W.; Hayashi,T.; Kita,K.; Takahashi,T.; Kuge,S.; Naganuma,A. “siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells” J. Toxicol. Sci. , 2007. 32,581-583.
  • Kadowaki, O.; Ogasawara,A.; Watanabe,T.; Mikami,T.; Matsumoto,T.; Misawa,Y.; Matahira,Y.; Sakai,K. “Chitohexose Induce the Yeast Proliferation of Candida albicansBiol. Pharm. Bull. ,2007, 30, 583-584.
  • Ogasawara, A.; Komaki,N.; Akai,H.; Hori,K.; Watanabe,H.; Watanabe,T.; Mikami,T.; Matsumoto,T. “Hyphal formation of Candida albicans is inhibited by salivary mucinBiol. Pharm. Bull. , 2007, 30, 284-286.
  • Watanabe,T.; Ogasawara,A.; Mikami,T.; Matsumoto,T.; “Hyphal formation of Candida albicans is controlled by electron transfer system” Biochem. Biophys. Res.Commun. , 2006, 348 ,206-211.
  • Konno,N., Ishii,M.; Nagai,A.; Watanabe,T.; Ogasawara,A.; Mikami,T.; Matsumoto,T. “Mechanism of Candida albicans Transformation in Response to Changes of pH” Biol. Pharm. Bull. , 2006, 29, 923-926.
  • Okazaki,S., Naganuma,A.; Kuge,S.; “Peroxiredoxin-mediated redox regulation of the nuclear localization of Yap1, a transcription factor in budding yeast” Antioxid. Redox Signal. , 2005, 7, 327-334.
  • Ohka,S.; Matsuda,N.; Tohyama,K.; Oda,T.; Morikawa,M.; Kuge,S.; Nomoto,A. “Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome” J. Virol. , 2004, 78, 7186-7198.
  • Maeta,K.; Izawa,S.; Okazaki,S.; Kuge,S.; Inoue,Y. “Activity of the Yap1 transcription factor in Saccharomyces cerevisiae is modulated by methylglyoxal, a metabolite derived from glycolysis” Mol. Cell Biol. , 2004, 24, 8753-8764.
  • Naganuma,A.; Furuchi,T.; Miura,N.; Hwang,G.W.; Kuge,S. “Investigation of intracellular factors involved in methylmercury toxicit” Tohoku J. Exp. Med. , 2002, 196, 65-70.
  • Isoyama,T.; Kuge,S.; Nomoto,A. “The core protein of hepatitis C virus is imported into the nucleus by transport receptor Kap123p but inhibits Kap121p-dependent nuclear import of yeast AP1-like transcription factor in yeast cells” J. Biol. Chem. , 2002, 277, 39634-39641.

08 Infection and Host Defense

Research Projects

  • Ishikawa,T., Itoh,F., Yoshida,S., Saijo,S., Matsuzawa,T., Gonoi,T., Saito,T., Okawa,Y., Shibata,N., Miyamoto,T., Yamasaki,S. “Identification of Distinct Ligands for the C-type Lectin Receptors Mincle and Dectin-2 in the Pathogenic Fungus Malassezia
    Cell Host & Microbe 2013, 13, 477-488.
  • Shibata,N., Kobayashi,H., Suzuki,S. “Immunochemistry of pathogenic yeast, Candida species, focusing on mannan”
    Proc. Jpn. Acad. Ser. B. 2012, 88, 250-265.
  • Shibata,N., Kobayashi,H., Suzuki,S. “Immunochemistry of pathogenic yeast, Candida species, focusing on mannan”
    FEBS J. 2012, 279, 1844-1856.
  • Takahara,K., Arita,T., Tokieda,S., Shibata,N., Okawa,Y., Tateno,H., Hirabayashi,J., Inaba,K. “Difference in fine specificity to polysaccharides of C. albicans mannoprotein between mouse SIGNR1 and human DC-SIGN”
    Infect. Immun. 2012, 80, 1699-1706.
  • Shibata,N.; Okawa,Y. “Chemical structure of -galactofuranose-containing polysaccharide and O-linked oligosaccharides obtained from the cell wall of pathogenic dematiaceous fungusFonsecaea pedrosoi
    Glycobiology 2011, 21, 69-81.
  • Shibata,N.; Okawa,Y. “Conformational analysis of -1,2-linked mannobiose to mannoheptaose, specific antigen of pathogenic yeast Candida albicans
    Chem. Pharm. Bull. 2010, 58, 1386-1390.
  • Shibata,N.; Okawa,Y. “Enzymatic synthesis of new oligosaccharides using mannosyltransferases from Candida species and their NMR assignments”
    Biol. Pharm. Bull. 2010, 33, 895-899.
  • Koyama,T.: Makita,M.: Shibata,N.: Okawa,Y. “Influence of oxidative and osmotic stresses on the structure of the cell wall mannan of Candida albicans serotype A”
    Carbohydr Res. 2009, 344, 2195-2200.
  • Shibata,N.; Saitoh,T.; Tadokoro,Y.; Okawa,Y. “The cell wall galactomannan antigen fromMalassezia furfur and Malassezia pachydermatis contains -1,6-linked linear galactofuranosyl residues and its detection has diagnostic potential”
    Microbiology-SGM 2009, 155, 3420-3429.
  • Goto,K.; Suzuki,A.; Shibata,N.; Okawa,Y. “Some properties of -1,2-mannosyltransferases related to the biosynthesis of the acid-labile oligomannosyl side chains in Candida albicansNIH B-792 strain cells”
    Biol. Pharm. Bull. 2009, 32, 1921-1923.
  • Oyamada,H.; Ogawa,Y.; Shibata,N.; Okawa,Y.; Suzuki,S.; Kobayashi,H. “Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes”
    Arch. Microbiol. 2008, 189, 483-490.
  • Shibata,N.; Suzuki,A.; Kobayashi,H.; Okawa,Y. “Chemical structure of the cell-wall mannan of Candida albicans serotype A and its difference in yeast and hyphal forms”
    Biochem. J. 2007, 404, 365-372.
  • Shibata,N.; Okawa,Y. “Structure of fungal cell wall polysaccharides”
    Jpn. J. Med. Mycol. 2006, 47, 179-184.
  • Shibata,N.; Okanuma,N.; Hirai,K.; Arikawa,K.; Kimura,M.; Okawa,Y. “Isolation, characterization, and molecular cloning of lipolytic enzyme secreted from Malassezia pachydermatis
    FEMS Microbiol. Lett. 2006, 256, 137-144.
  • Kobayashi,H.; Oyamada,H.; Matsuda,K.; Shibata,N.; Suzuki,S. “Distribution of antigenic oligomannosyl side chains in the cell wall mannans of several strains of Candida tropicalis
    Arch. Microbiol. 2003, 180, 76-80.
  • Shibata,N.; Kobayashi,H.; Okawa,Y.; Suzuki,S. “Existence of novel -1,2 linkage-containing side chain in the mannan of Candida lusitaniae, antigenically related to Candida albicans serotype A”
    Eur. J. Biochem. 2003, 270, 2565-2575.
  • Okawa,Y.; Monma,K.; Shibata,N.; Kobayashi,H.; Yamada,Y. “A new mannoheptaose containing and -(1-2) linkages isolated from the mannan of Torulaspora delbrueckii: ELISA inhibition studies”
    Carbohydr. Res. 2003, 338, 1175-1182.

09 Environmental and Health Sciences

Research Projects

  • S. Sakaguchi, S. Takahashi, T. Sasaki, T. Kumagai, K. Nagata, Progression of Alcoholic and Non-alcoholic Steatohepatitis: Common Metabolic Aspects of Innate Immune System and Oxidative Stress. Drug Metab Pharmacokinet. 226:30-46 (2011)
  • W. Sato, H. Suzuki, T. Sasaki, T. Kumagai, S. Sakaguchi, M. Mizugaki, S. Miyairi, Y. Yamazoe, K. Nagata, Construction of a system that simultaneously evaluates CYP1A1 and CYP1A2 induction in a stable human-derived cell line using a dual reporter plasmid. Drug Metab Pharmacokinet. 25: 180-189 (2010)
  • H. Suzuki, T. Sasaki, T. Kumagai, S. Sakaguchi, K. Nagata, Malondialdehyde-modified low density lipoprotein (MDA-LDL)-induced cell growth was suppressed by polycyclic aromatic hydrocarbons (PAHs). J Toxicol Sci. 35: 137-147 (2010)
  • K. Aoyama, K. Yoshinari, H.J. Kim, K. Nagata. Y. Yamazoe, Simultaneous expression of plural forms of human cytochrome P450 at desired ratios in HepG2 cells: adenovirus-mediated tool for cytochrome P450 reconstitution. Drug Metab Pharmacokinet. 24:209-217 (2009).
  • T. Toriyabe, T. Takada, Y. Aratsu, T. Matsubara, Y. Yoshinari, K. Nagata, Y. Yamazoe, Unveiling a new essential cis-element for the transactivation of the CYP3A4 gene by xenobiotics. Mol Pharmacol. 75: 677-684 (2009).
  • T. Matsubara, K. Yoshinari, K. Aoyama, M. Sugawara, T. Sekiya K. Nagata, Y. Yamazoe, Role of vitamin D receptor in the lithocholic acid-mediated CYP3A induction in vitro and in vivo. Drug Metab Dispos. 36: 2058-2063 (2008).
  • Y. Kamiyama, T. Matsubara, K. Yoshinari, K. Nagata, H. Kamimura, Y. Yamazoe, Role of human hepatocyte nuclear factor 4alpha in the expression of drug-metabolizing enzymes and transporters in human hepatocytes assessed by use of small interfering RNA. Drug Metab Pharmacokinet. 22:287-298 (2007).
  • K. Kojima, K. Nagata, T. Matsubara, Y. Yamazoe, Broad but distinct role of pregnane x receptor on the expression of individual cytochrome p450s in human hepatocytes. Drug Metab Pharmacokinet. 22:276-286 (2007).
  • R. Ueda, H. Iketaki, K. Nagata, S. Kimura, F.J. Gonzalez, K. Kusano, T. Yoshimura, Y. Yamazoe, A common regulatory region functions bidirectionally in transcriptional activation of the human CYP1A1 and CYP1A2 genes., Mol. Pharmacol., 69:1924-1930 (2006)
  • T. Kumagai, H. Tsutsumi, N. Ogawa, S. Naito, K. Ebina, K. Yokota, K. Nagata, Oxidized low-density lipoprotein-binding specificity of the Asp-hemolysin-related synthetic peptides from Aspergillus fumigatus. Biol Pharm Bull29: 2181-2186 (2006).

10 Pharmacology

Research Projects

  • Nakagawasai,O.; Yamada,K.; Nemoto,W.; Fukahori,M.; Tadano,T.; Tan-No,K. Liver hydrolysate assists in the recovery from physical fatigue in a mouse model.
    J. Pharmacol. Sci. 2013, 123, 328-335.
  • Nemoto,W.; Nakagawasai,O.; Yaoita,F.; Kanno,SI.; Yomogida,S.; Ishikawa,M.; Tadano,T.; Tan-No,K. Angiotensin II produces nociceptive behavior through spinal AT1 receptor-mediated p38 mitogen-activated protein kinase activation in mice.
    Mol. Pain 2013, 9, 38.
  • Niijima-Yaoita,F.; Tsuchiya,M.; Saito,H.; Nagasawa,Y.; Murai,S.; Arai,Y.; Nakagawasai,O.; Nemoto,W.; Tadano,T.; Tan-No,K. Influence of a long-term powdered diet on the social interaction test and dopaminergic systems in mice.
    Neurochem. Int. 2013, 63, 309-15.
  • Taniguchi,R.; Nakagawasai,O.; Tan-No,K.; Yamadera,F.; Nemoto,W.; Sato,S.; Yaoita,F.; Tadano,T. Combined low calcium and lack magnesium is a risk factor for motor deficit in mice.
    Biosci. Biotechnol. Biochem. 2013, 77, 266-70.
  • Mitazaki,S.; Hashimoto,M.; Matsuhashi,Y.; Honma,S.; Suto,M.; Kato,N.; Nakagawasai,O.; Tan-No,K.; Hiraiwa,K.; Yoshida,M.; Abe S. Interleukin-6 modulates oxidative stress produced during the development of cisplatin nephrotoxicity.
    Life Sci. 2013, 92, 694-700.
  • Yabuki,Y.; Nakagawasai,O.; Moriguchi,S.; Shioda,N.; Onogi,H.; Tan-No,K.; Tadano T, Fukunaga K. Decreased CaMKII and PKC activities in specific brain regions are associated with cognitive impairment in neonatal ventral hippocampus-lesioned rats.
    Neuroscience 2013, 234, 103-115.
  • Nemoto,W.; Sato,T.; Nakagawasai,O.; Yaoita,F.; Silberring,J.; Tadano,T.; Tan-No,K. Phenylmethanesulfonyl fluoride, a serine protease inhibitor, suppresses naloxone-precipitated withdrawal jumping in morphine-dependent mice.
    Neuropeptides 2013, 47, 187-191.
  • Oba,A.; Nakagawasai,O.; Onogi,H.; Nemoto,W.; Yaoita,F.; Arai,Y.; Tan-No,K.; Tadano,T. Chronic fluvoxamine treatment changes 5-HT(2A/2C) receptor-mediated behavior in olfactory bulbectomized mice.
    Life Sci. 2013, 92, 119-124.
  • Sato,S.; Nakagawasai,O.; Hayashi,T.; Oikawa,A.; Yaoita,F.; Tan-No,K.; Tadano,T.; Suzuki,T. Enhanced behavioral response to serotonin-related agonists in postweaning protein malnourished mice.
    Biol. Pharm. Bull. 2012, 35, 1697-1702.
  • Sato,S.; Nakagawasai,O.; Tan-No,K.; Niijima,F.; Suzuki,T.; Tadano,T. Executive functions of postweaning protein malnutrition in mice.
    Biol. Pharm. Bull. 2011, 34, 1413-1417.
  • Onogi,H.; Nakagawasai,O.; Sato,A.; Mitazaki,S.; Nakaya,K.; Watanabe,K.; Niijima-Yaoita,F.; Tan-No,K.; Arai,Y.; Kikuchi,T.; Tadano,T. p-Hydroxyamphetamine causes prepulse inhibition disruption in mice: contribution of serotonin neurotransmission.
    Behav. Brain Res. 2011, 224, 159-165.
  • Watanabe,S.; Tan-No,K.; Tadano,T.; Higashi,H. Intraplantar injection of gangliosides produces nociceptive behavior and hyperalgesia via a glutamate signaling mechanism.
    Pain 2011, 152, 327-334.
  • Sato,A.; Nakagawasai,O.; Tan-No,K.; Onogi,H.; Niijima,F.; Tadano,T. Influence of olfactory bulbectomy on maternal behavior and dopaminergic function in nucleus accumbens in mice.
    Behav. Brain Res. 2010, 215, 141-145.
  • Onogi,H.; Nakagawasai,O.; Tan-No,K.; Mitazaki,S.; Sato,A.; Nakaya,K.; Niijima,F.; Arai,Y.; Kikuchi,T.; Tadano,T. p-Hydroxyamphetamine causes prepulse inhibition disruptions in mice: contribution of dopamine neurotransmission.
    Behav. Brain Res. 2010, 214, 349-356.
  • Niijima,F.; Saito,H.; Murai,S.; Arai,Y.; Nakagawasai,O.; Tan-No,K.; Watanabe,H.; Hiraga,H.; Tadano,T. Effects of atomoxetine on levels of monoamines and related substances in discrete brain regions in mice intermittently deprived of rapid eye movement sleep.
    Biol. Pharm. Bull. 2010, 33, 617-621.
  • Sato,A.; Nakagawasai,O.; Tan-No,K.; Onogi,H.; Niijima,F.; Tadano,T. Effect of non-selective dopaminergic receptor agonist on disrupted maternal behavior in olfactory bulbectomized mice.
    Behav. Brain Res. 2010, 210, 251-256.
  • Tan-No,K.; Sato,T.; Shimoda,M.; Nakagawasai,O.; Niijima,F.; Kawamura,S.; Furuta,S.;
    Sato,T.; Satoh,S.; Silberring,J.; Terenius,L.; Tadano,T. Suppressive effects by cysteine protease inhibitors on naloxone-precipitated withdrawal jumping in morphine-dependent miceNeuropeptides 2010, 44, 279-283.
  • Nakagawasai,O.; Onogi,H.; Mitazaki,S.; Sato,A.; Watanabe,K.; Saito,H.; Murai,S.; Nakaya,K.; Murakami,M.; Takahashi,E.; Tan-No,K.; Tadano.T. Behavioral and neurochemical characterization of mice deficient in the N-type Ca2+ channel 1B subunit.
    Behav. Brain Res. 2010, 208, 224-230.
  • Niijima,F.; Saito,H.; Murai,S.; Arai,Y.; Nakagawasai,O.; Tan-No,K.; Watanabe,H.; Hiraga,H.; Tadano,T. Effects of atomoxetine on levels of monoamines and related substances in discrete brain regions in mice intermittently deprived of rapid eye movement sleep.
    Biol. Pharm. Bull. 2010, 33, 617-621.
  • Tan-No,K.; Shimoda,M.; Watanabe,K.; Nakagawasai,O.; Niijima,F.; Kanno,S.; Ishikawa,M.; Bakalkin,G.; Tadano,T. Involvement of the p53 tumor-suppressor protein in the development of antinociceptive tolerance to morphine.
    Neurosci. Lett. 2009, 450, 365-368.
  • Tan-No,K.; Takahashi,H.; Nakagawasai,O.; Niijima,F.; Sakurada,S.; Bakalkin,G.; Terenius,L.; Tadano,T. Nociceptive behavior induced by the endogenous opioid peptides dynorphins in uninjured mice: evidence with intrathecal N-ethylmaleimide inhibiting dynorphin degradation.
    Int. Rev. Neurobiol. 2009, 85, 191-205. Nakagawasai,O.; Oba,A.; Sato,A.; Arai,Y.; Mitazaki,S.; Onogi,H.; Wakui,K.; Niijima,F.; Tan-No,K.; Tadano,T. Subchronic stress-induced depressive behavior in ovariectomized mice.
    Life Sciences 2009, 84, 512-516. Tan-No,K.; Shimoda,M.; Sugawara,M.; Nakagawasai,O.; Niijima,F.; Watanabe,H.; Furuta,S.; Sato,T.; Satoh,S.; Arai,Y.; Kotlinska,J.; Silberring,J.; Terenius,L.; Tadano,T. Cysteine protease inhibitors suppress the development of tolerance to morphine antinociception.
    Neuropeptides 2008, 42, 239-244.
  • Nakagawasai,O.; Murata,A.; Arai,Y.; Ohba,A.; Wakui,K.; Mitazaki,S.; Niijima,F.; Tan-No,K.; Tadano,T. Enhanced head-twitch response to 5-HT-related agonists in thiamine-deficient mice.
    J. Neural. Transm. 2007, 114, 1003-1010.
  • Nakagawasai,O.; Yamadera,F.; Iwasaki,K.; Asao,T.; Tan-No,K.; Niijima,F.; Arai,H.; Tadano,T. Preventive effect of kami-untan-to on performance in the forced swimming test in thiamine-deficient mice: Relationship to functions of catecholaminergic neurons.
    Behav. Brain Res. 2007, 177, 315-321.
  • Niijima,F.; Nakagawasai,O.; Tan-No,K.; Tadano,T. Inhibitory effects of methylphenidate and atomoxetine on jumping behavior induced by intermittent rapid eye movement (REM) sleep deprivation treatment in mice.
    Biogenic Amines 2006, 20, 99-111.
  • Nakagawasai,O.; Yamadera,F.; Sato,S.; Taniguchi,R.; Hiraga,H.; Arai,Y.; Murakami,H.; Mawatari,K.; Niijima,F.; Tan-No,K.; Tadano,T. Alterations in cognitive function in prepubertal mice with protein malnutrition: Relationship to changes in choline acetyltransferase.
    Behav. Brain Res. 2006, 167, 111-117.
  • Tan-No,K.; Taira,A.; Nakagawasai,O.; Niijima,F.; Demuth,H-U.; Silberring,J.; Terenius,L.; Tadano,T. Differential effects of N-peptidyl-O-acyl hydroxylamines on dynorphin-induced antinociception in the mouse capsaicin test.
    Neuropeptides 2005, 39, 569-573.
  • Tan-No,K.; Takahashi,H.; Nakagawasai,O.; Niijima,F.; Sato,T.; Satoh,S.; Sakurada,S.; Marinova,Z.; Yakovleva,T.; Bakalkin,G.; Terenius,L.; Tadano,T. Pronociceptive role of dynorphins in uninjured animals: N-ethylmaleimide-induced nociceptive behavior mediated through inhibition of dynorphin degradation.
    Pain 2005, 113, 301-309.
  • Laplante,F.; Nakagawasai,O.; Srivastava,LK.; Quirion,R. Alterations in behavioral responses to a cholinergic agonist in post-pubertal rats with neonatal ventral hippocampal lesions: relationship to changes in muscarinic receptor.
    Neuropsychopharmacology 2005, 30, 1076-1087.
  • Nakagawanai,O.; Yamadera,F.; Iwasaki,K.; Arai,H.; Taniguchi,R.; Tan-No,K.; Sasaki,H.; Tadano,T. Effect of Kami-Untan-To on the impairment of learning and memory induced by thiamine-deficient feeding in mice.
    Neuroscience 2004, 125, 233-241.
  • Tan-No,K.; Esashi,A.; Nakagawasai,O.; Niijima,F.; Sakurada,C.; Sakurada,T.; Bakalkin,G.; Terenius,L.; Tadano,T. Nociceptive behavior induced by poly-L-lysine and other basic compounds involves the spinal NMDA receptors.
    Brain Res. 2004, 1008, 49-53.
  • Tan-No,K.; Niijima,F.; Nakagawasai,O.; Sato,T.; Satoh,S.; Tadano,T. Development of tolerance to the inhibitory effect of loperamide on gastrointestinal transit in mice.
    Eur. J. Pharma. Sci. 2003, 20, 357-363.
  • Nakagawasai,O.; Hozumi,S.; Tan-No,K.; Niijima,F.; Arai,Y.; Yasuhara,H.; Tadano,T. Immunohistochemical fluorescence intensity reduction of brain somatostatin in the impairment of learning and memory-related behaviour induced by olfactory bulbectomy.
    Behav. Brain Res. 2003, 142, 63-67.
  • Nakagawasai,O.; Tadano,T.; Arai,Y.; Hozumi,S.; Oba,A.; Tan-No,K.; Yasuhara,H.; Kisara,K.; Oreland,L. Enhancement of 5-hydroxytryptamine-induced head-twitch response after olfactory bulbectomy.
    Neuroscience 2003, 117, 1017-1023.
  • Tan-No,K.; Esashi,A.; Nakagawasai,O.; Niijima,F.; Tadano,T.; Sakurada,C.; Sakurada,T.; Bakalkin,G.; Terenius,L.; Kisara,K. Intrathecally administered big dynorphin, a prodynorphin-derived peptide, produces nociceptive behavior through an N-methyl-D-aspartate receptor mechanism.
    Brain Res. 2002, 952, 7-14.

11 Physiology and Anatomy

Research Projects

  • Aoki,Y.; Mizoguchi,H.; Watanabe,C.; Takeda,K.; Sakurada,T.; Sakurada,S. “Potential involvement of μ-opioid receptor dysregulation on the reduced antinociception of morphine in the inflammatory pain state in mice”
    J. Pharmacol. Sci. 2014, 124, 258-266.
  • Aoki,Y.; Mizoguchi,H.; Watanabe,C.; Sakurada,T.; Sakurada,S. “Differential alternation of the antinociceptive effect of narcotic analgesics on the inflammatory pain state”
    Neurosci. Lett. 2014, 560, 122-125.
  • Mizoguchi,H.; Kon-no,T.; Watanabe,H.; Watanabe,C.; Yonezawa,A.; Sato,T.; Sakurada,T.; Sakurada,S. “Involvement of spinal release of α-neo-endorphin on the antinociceptive effect of TAPA”
    Peptides 2013, 50, 139-144.
  • Mizoguchi,H.; Watanabe,C.; Higashiya,T.; Takeda,S.; Moriyama,K.; Aoki,Y.; Kon-no,T.; Takagi,H.; Yonezawa,A.; Sato,T.; Sakurada,T.; Sakurada,S. “Distinct physiological role of amidino-TAPA-sensitive and DAMGO-insensitive α-opioid receptor splice variants in the mouse spinal cord”
    Eur. J. Pharmacol. 2013, 711, 80-86.
  • Watanabe,C.; Mizoguchi,H.; Bagetta,G.; Sakurada,S. “The involvement of the spinal release of glutamate and nitric oxide in peripheral noxious stimulation-induced pain-related behaviors -Study in mouse spinal microdialysis”
    Neurosci. Lett. 2012, 515, 111-114.
  • Mizoguchi,H.; Watanabe,C.; Sakurada,T.; Sakurada,S. “New vistas in opioid control of pain”
    Curr. Opin. Pharmacol. 2012, 12, 87-91.
  • Mizoguchi,H.; Komatsu,T.; Iwata,Y.; Watanabe,C.; Watanabe,H.; Orito,T.; Katsuyama,S.; Yonezawa,A.; Onodera,K.; Sakurada,T.; Sakurada,S. “Partial involvement of NMDA receptors and glial cells in the nociceptive behaviors induced by intrathecally administered histamine”
    Neurosci. Lett. 2011, 495, 83-87.
  • Mizoguchi,H.; Bagetta,G.; Sakurada,T.; Sakurada,S. “Dermorphin tetrapeptide analogs as potent and long-lasting analgesics with pharmacological profiles distinct from morphine”
    Peptides 2011, 32, 421-427.
  • Mizoguchi,H.; Komatsu,T.; Iwata,Y.; Watanabe,C.; Watanabe,H.; Orito,T.; Katsuyama,S.; Yonezawa,A.; Onodera,K.; Sakurada,T.; Sakurada,S. “Involvement of glial cells in the nociceptive behaviors induced by a high-dose of histamine administered intrathecally”
    Eur. J. Pharmacol. 2011, 653, 21-25.
  • Mizoguchi,H.; Watanabe,C.; Higashiya,T.; Takeda,S.; Moriyama,K.; Yonezawa,A.; Sato,T.; Komatsu,T.; Sakurada,T.; Sakurada,S. “Involvement of mouse μ-opioid receptor splice variants in the spinal antinociception induced by the dermorphin tetrapeptide analog amidino-TAPA”
    Eur. J. Pharmacol. 2011, 651, 66-72.
  • Mizoguchi,H.; Watanabe,C.; Osada,S.; Yoshioka,M.; Aoki,Y.; Natsui,S.; Yonezawa,A.; Kanno,S.; Ishikawa,M.; Sakurada,T.; Sakurada,S. “Lack of a rewarding effect and a locomotor-enhancing effect of the selective μ-opioid receptor agonist amidino-TAPA”
    Psychopharmacology 2010, 212, 215-225.
  • Watanabe,C.; Mizoguchi,H.; Yonezawa,A.; Sakurada,S. “Characterization of intrathecally administered hemokinin-1-induced nociceptive behaviors in mice”
    Peptides 2010, 31, 1613-1616.
  • Mizoguchi,H.; Watanabe,C.; Yonezawa,A.; Sakurada,S. “New therapy for neuropathic pain”
    Int. Rev. Neurobiol. 2009, 85, 249-260.
  • Sakurada,S.; Sawai,T.; Mizoguchi,H.; Watanabe,H.; Watanabe,C.; Yonezawa,A.; Morimoto,M.; Sato,T.; Komatsu,T.; Sakurada,T. “Possible involvement of dynorphin A release via μ1-opioid receptor on supraspinal antinociception of endomorphin-2”
    Peptides 2008, 29, 1554-1560.
  • Watanabe,C.; Orito,T.; Watanabe,H.; Mizoguchi,H.; Yonezawa,A.; Yanai,K.; Mobarakeh,J.I.; Onodera,K.; Sakurada,T.; Sakurada,S. “Intrathecal high-dose histamine induces spinally-mediated behavioral responses through a polyamine site of NMDA receptors”
    Eur. J. Pharmacol. 2008, 581, 54-63.
  • Nakayama,D.; Watanabe,H.; Watanabe,C.; Mizoguchi,H.; Sakurada,T.; Sakurada,S. “A Tyr-W-MIF-1 analog containing D-Pro2 discriminates among antinociception in mice mediated by different classes of μ-opioid receptors”
    Eur. J. Pharmacol. 2007, 563, 109-116.
  • Mizoguchi,H.; Watanabe,C.; Watanabe,H.; Moriyama,K.; Sato,B.; Ohwada,K.; Yonezawa,A.; Sakurada,T.; Sakurada,S. “Involvement of endogenous opioid peptides in the antinociception induced by the novel dermorphin tetrapeptide analog amidino-TAPA”
    Eur. J. Pharmacol. 2007, 560, 150-159.
  • Watanabe,H.; Mizoguchi,H.; Orito,T.; Katsuyama,S.; Yonezawa,A.; Watanabe,C.; Sakurada,T.; Sakurada,S. “Possible involvement of endogenous nociceptin/orphanin FQ in the pain-related behavioral responses induced by its own metabolite, nociceptin/orphanin FQ(14-17)”
    Peptides 2007, 28, 670-677.

12 Pathophysiology

Research Projects

  • Okuyama K, Dobashi K, Miyasaka T, Yamazaki N, Kikuchi T, Sora I, Takayanagi M, Kita H, Ohno I.
    The involvement of glucocorticoids in psychological stress-induced exacerbations of experimental allergic asthma.
    Int Arch Allergy Immunol, 2014 (in press)
  • Inokuchi J, Nagafuku M, Ohno I, Suzuki A. Heterogeneity of gangliosides among T cell subsets.
    Cell Mol Life Sci 70:3067-3075, 2013
  • Okuyama K, Suenaga M, Furuk S, Kawano T, Ohkawara Y, Takayanagi M, Kikuchi T, Ohno I.
    Contribution of CD4+ T cells and dendritic cells to female-dominant antigen-induced Th2 cytokine production by bronchial lymph node cells.
    Int Arch Allergy Immunol 161:58-65, 2013
  • Okuyama K, Kashimura T, Kawano T, Ohkawara Y, Takayanagi M, Kikuchi T, Ohno I. Higher sensitivity of male CD4+ T cells to suppressive effects of CD8+ T cells on IL-5 production compared with female CD4+ T cells.
    Int Arch Allergy Immunol 158:35-41, 2012
  • Nagafuku M, Okuyama K, Onimaru Y, Suzuki A, Odagiri Y, Yamashita T, Iwasaki K, Fujiwara M, Takayanagi M, Ohno I, Inokuchi JI. CD4 and CD8 T cells require different membrane gangliosides for activation.
    Proc Natl Acad Sci USA 109:E336-E342, 2012
  • Okuyama K, Ide S, Sakurada S, Komatsu H, Sora I, Tamura G, Ohkawara Y, Takayanagi M, Ohno I.
    μ-opioid receptor-mediated alterations of allergen-induced immune responses of bronchial lymph node cells in a murine model of stress asthma.
    Allergol Int 61:245-258, 2012
  • Okuyama,K.; Hamanaka,Y.; Kawano,T.; Ohkawara,Y.; Takayanagi,M.; Kikuchi,T.; Ohno,I. “T cell subsets related with a sex difference in IL-5 production.”
    Int. Arch. Allergy Immunol. 2011, 115, 21-26.
  • Wada,K.; Okuyama,K.; Ohkawara,Y.; Takayanagi,M.; Ohno,I.; “Sex differences in transcriptional regulation of IL-5 expression by bronchial lymph node cells in a mouse model of asthma.”
    Respirology 2010, 15, 629-635.
  • Suzuki,K.; Nishimaki,K.; Okuyama,K.; Katoh,T.; Yasujima,M.; Chihara,J.; Suwabe,A.; Shibata,Y.; Takahashi,C.; Takeda,H.; Ida,S.; Kaku,M.; Watanabe,A.; Nukiwa,T.; Niitsuma,K.; Kanemitsu,K.; Takayanagi,M.; Ohno,I. “Trends in antimicrobial susceptibility of Streptococcus pneumoniae in the Tohoku district of Japan: a longitudinal analysis from 1998 to 2007.”
    Tohoku J. Exp. Med. 2010, 220, 47-57.
  • Okuyama,K.; Wada,K.; Sakurada,S.; Mizoguchi,H.; Komatsu,H.; Sora,I.; Tamura,G.; Ohkawara,Y.; Takayanagi,M.; Ohno,I. “The involvement of μ-opioid receptors in the central nervous system in the worsening of allergic airway inflammation by psychological stress in mice.”
    Int. Arch. Allergy Immunol. 2010, 152, 342-352.
  • Takeda,M.; Ito,W.; Tanabe,M.; Ueki,S.; Kato,H.; Kihara.;J.; Tanigai,T.; Chiba,T.; Yamaguchi,K.; Kayaba,H.; Imai,Y.; Okuyama,K.; Ohno,I.; Sasaki,T.; Chihara,J. “Allergic airway hyperresponsiveness, inflammation, and remodeling do not develop in phosphoinositide 3-kinase gamma-deficient mice.”
    J. Allergy Clin. Immunol. 2009, 123, 805-812.
  • Okuyama,K.; Wada,K.; Chihara,J.; Takayanagi,M.; Ohno,I. “Sex-related splenocyte function in a murine model of allergic asthma.”
    Clin. Exp. Allergy 2008, 38, 1212-1219.
  • Ohwada,K.; Watanabe,K.; Okuyama,K.; Ohkawara,Y.; Sugaya,T.; Takayanagi,M.; Ohno,I. “The involvement of type 1a angiotensin II receptors in the regulation of airway inflammation in a murine model of allergic asthma.”
    Clin. Exp. Allergy 2007, 37, 1720-1727.
  • Nishimaki,K.; Okuyama,K.; Okada,S.; Hattori,T.; Takayanagi,M.; Ohno,I. “Prolongation of neutrophil survival by the culture supernatant of Pseudomonas aeruginosa.
    Respirology 2007, 12,664-669.
  • Okuyama,K.; Ohwada,K.; Sakurada,S.; Sato,N.; Sora,I.; Tamura,G.; Takayanagi,M.; Ohno,I. “The distinctive effects of acute and chronic psychological stress on airway inflammation in a murine model of allergic asthma.”
    Allergol. Int. 2007,56, 29-35.
  • Okuyama,K.; Yamashita,M.; Kitabatake,Y.; Kawamura,S.; Takayanagi,M.; Ohno,I. “Ciglitazone inhibits the antigen-induced leukotrienes production independently of PPARγ in RBL-2H3 mast cells.”
    Eur. J. Pharmacol. 2005, 521, 21-28.
  • Nishimaki,K.; Nawata,J.; Okada,S.; Hattori,T.; Ohno,I. “Neutrophil survival-enhancing activity in sputum from patients with diffuse panbronchiolitis.”
    Respir. Med. 2005, 99, 910-917.
  • Wang,W.; Masu,K.; Tamura,G.; Suzuki,K.; Ohwada,K.; Okuyama,K.; Shirato,K.; Takayanagi,M.; Ohno,I. “Inhibition of eosinophil survival by a selective inhibitor of phosphodiesterase 4 via the induction of apoptosis.”
    Biol. Pharm. Bull. 2005, 28, 515-519.
  • Kitabatake,Y.; Kawamura,S.; Yamashita,M.; Okuyama,K.; Takayanagi,M.; Ohno,I. “The expression of mRNA for calcitonin gene-related peptide receptors in a mucosal type mast cell line, RBL-2H3.”
    Biol. Pharm. Bull. 2004, 27,896-898.
  • Nishimaki,K.; Okada,S.; Miyamura,K.; Ohno,I.; Ashino,Y.; Sugawara,T.; Kondo,T.; Hattori,T. “The possible involvement of human herpesvirus type 6 in obliterative bronchiolitis after bone marrow transplantation.”
    Bone Marrow Transplant. 2003, 32, 1103-1105.
  • Miura,S.; Ohno,I.; Suzuki,J.; Suzuki,K.; Okada,S.; Okuyama,A.; Nawata,J.; Ikeda,J.; Shirato,K. “Inhibition of matrix metalloproteinases prevents cardiac hypertrophy induced by beta-adrenergic stimulation in rats.”
    J. Cardiovasc. Pharmacol. 2003, 42, 174-181.
  • Kumagai,K.; Ohno,I.; Imai,K.; Nawata,J.; Hayashi,K.; Okada,S.; Senoo,H.; Hattori,T.; Shirato,K. “The involvement of matrix metalloproteinases in basement membrane injury in a murine model of acute allergic airway inflammation.”
    Clin. Exp. Allergy 2002, 32, 1527-1534.
  • Okada,S.; Kikuchi,M.; Hasegawa,H.; Ishikawa,M.; Ohno,I.; Kaku,M.; Hattori,T. “Delayed recovery of effector memory CD4+ T cells by highly active antiretroviral therapy in a patient with HIV-1 infection.”
    Tohoku J. Exp. Med. 2002, 196, 213-218.
  • Masu,K.; Ohno,I.; Suzuki,K.; Okada,S.; Hattori,T.; Shirato,K. “Proliferative effects of eosinophil lysates on cultured human airway smooth muscle cells”
    Clin. Exp. Allergy 2002, 32, 595-601.

13 Natural Product Chemistry

Research Projects

  • Kapojos, M.M.; Lee, J.-S.; Oda, T.; Nakazawa, T.; Takahashi, T.; Ukai, K.; Mangindaan, R.E.P.; Rotinsulu, H.; Wewengkang, D.S.; Tsukamoto, S.; Kobayashi, H.; Namikoshi, M. “Two Unprecedented Cembrene-Type Terpenes from an Indonesian Soft Coral Sarcophyton sp.”
    Tetrahedron, 2010, 66, 641-645.
  • Oda, T. ; Xu, J.; Ukai, K.; Nakazawa, T.; Namikoshi, M.; “12′-Hydroxyl group remarkably reduces Roridin E cytotoxicity”
    Mycoscience, 2010, 51, 317-320.
  • Ukai, K.; Nakazawa, T.; Namikoshi, M. “Isolation of an Autotomy-Promoting Factor fromAsterias amurensis
    Echinoderms: Proceedings from the 12th International Echinoderm Conference, Taylor & Francis, 2010, 547-549.
  • Oda, T.; Lee, J.-S.; Sato, Y.; Kabe, Y.; Sakamoto, S.; Handa, H.; Mangindaan, R.E.P.; Namikoshi M. ” Inhibitory effect of N,N-Didesmethylgrossularine-1 on inflammatory cytokine production in Lipopolysaccharide-stimulated RAW 264.7 cells”
    Mar. Drugs, 2009, 7, 589-599.
  • Wang, W.; Takahashi, O.; Oda,T.; Nakazawa, T.; Ukai, K.; Mangindaan, R.E.P.; Rotinsulu, H.; Wewengkang, D.S.; Kobayashi, H.; Tsukamoto, S.; Namikoshi, M. “Lissoclibadins 8-14, polysulfur dopamine-derived alkaloids from the colonial ascidian Lissoclinum cf. badium
    Tetrahedron, 2009, 65, 9598-9603.
  • Wang, W.; Lee, J.-S.; Nakazawa, T.; Ukai, K.; Mangindaan, R.E.P.; Wewengkang, D.S.; Rotinsulu, H.; Kobayashi, H.; Tsukamoto, S.; Namikoshi M. “(25S)-Cholesten-26-oic acid derivatives from an Indonesian soft coral Minabea sp.”
    Steroids, 2009, 74, 758-760.
  • Namikoshi, M.; Xu, J. “Filamentous Fungi in the Marine Environment: Chemical Ecology”
    Fungi from different environment, edited by Misra, J.K.; Deshmush, S.K., 2009, Science Publishers 81-118.
  • Xu, J.; Nakazawa, T.; Ukai, K.; Kobayashi, H.; Mangindaan, R.E.P.; Wewengkang, D.S.; Rotinsulu, H.; Namikoshi, N. “Tetrahydrobostrycin and 1-Deoxytetrahydro -bostrycin, Two New Hexahydroanthrone Derivatives, from a Marine-derived Fungus Aspergillus sp.”
    J. Antibiot. , 2008, 61, 415-419.
  • Kapojos, M.M.; Mangindaan, R.E.P.; Nakazawa, T.; Oda, T.; Ukai, K.; Namikoshi, M.; “Three New Nardosinane Type Sesquiterpenes from an Indonesian Soft Coral Nephthea sp.”
    Chem. Pharm. Bull. , 2008, 56, 332-334.
  • Kito, K.; Ookura, R.; Yoshida, S.; Namikoshi, M.; Ooi, T.; Kusumi, T. “New cytotoxic 14-membered macrolides from marine-derived fungus Aspergillus ostianus
    Org. Letters, 2008, 10, 225-228.
  • Ookura, R.; Kito, K.; Ooi, T.; Namikoshi, M.; Kusumi T. “Structure Revision of Circumdatins A and B, Benzodiazepine Alkaloids Produced by Marine Fungus Aspergillus ostianus, by X-ray Crystallography”
    J. Org. Chem. , 2008, 73, 4245-4247.
  • Oda, T.; Wang, W.; Fujita, A.; Mochizukia, M.; Ukai, K.; Namikoshi, M. “Promotion of IL-8 production in PMA-stimulated HL-60 cells by sesquiterpene quinines from a marine sponge,Hippospongia sp.”
    J. Nat. Med. , 2007, 61, 434-437.
  • Nakazawa, T.; Xu, J.; Nishikawa, N.; Oda, T.; Fujita, A.; Ukai, K.; Mangindaan, R.P.M.; Rotinsulu, H.; Kobayashi, H.; Namikoshi, M. “Lissoclibadins 4-7, Polysulfur Aromatic Alkaloids from the Indonesian Ascidian Lissoclinum cf. badium
    J. Nat. Prod. , 2007, 70, 439-442.
  • Oda, T.; Kamoshita, K.; Maruyama, S.; Masuda, K.; Nishimoto, M.; Xu, J.: Ukai, K.; Mangindaan, R.E.P.; Namikoshi M. “Cytotoxicity of Lissoclibadins and Lisso -clinotoxins, Isolated from a Tropical Ascidian Lissoclinum cf. badium, against Human Solid-tumor-derived Cell Lines”
    Biol. Pharm. Bull. , 2007, 30, 385-387.
  • Wang, W.; Oda, T.; Fujita, A.; Mangindaan, R.E.P.; Nakazawa, T.; Ukai, K.; Kobayashi,
    H.; Namikoshi M. “Three new sulfur-containing alkaloids, polycarpaurines A, B, and C, from an Indonesian ascidian Polycarpa aurata
    Tetrahedron, 2007, 63, 409-412.
  • Wang, W.; Namikoshi, M.; “Bioactive Nitrogenous Metabolites from Ascidians”
    Heterocycles, 2007, 74, 53-88.
  • Oda, T.; Fujita, A.; Xu, J.; Mochizuki, M.; Ukai, K.; Namikoshi, M. “Effect of Melophlins on Colony Formation of Chinese Hamster V79 Cells and IL-8 Production in PMA-stimulated HL-60”
    Mar. Drugs, 2007, 5, 1-5.
  • Namikoshi, M.; Fujiwara, T.; Nishikawa, T.; Ukai, K. “Natural Abundance 14C Content of Dibutyl Phthalate (DBP) from Three Marine Algae”
    Mar. Drugs, 2006, 4, 290-297.

14 Pharmacognosy

Research Projects

  • Keiko Yamada, Toshihiro Murata, Kyoko Kobayashi, Toshio Miyase, Fumihiko Yoshizaki, “A lipase inhibitor monoterpene and monoterpene glycosides from Monarda punctata
    Phytochemistry, 71, 1884-1891 (2010)
  • Toshihiro Murata, Toshio Miyase, Fumihiko Yoshizaki, “Cyclic Spermidine Alkaloids and Flavone Glycosides from Meehania fargesii
    Chem. Pharm. Bull. , 58, 696-702 (2010)
  • Toshihiro Murata, Toshio Miyase, Fumihiko Yoshizaki, “Meehanines L-W, Spermidine Alkaloidal Glycosides from Meehania urticifolia
    J. Nat. Prod. , 72, 1937-1943 (2009)
  • Toshihiro Murata, Kenroh Sasaki, Kumiko Sato, Fumihiko Yoshizaki, Haruna Yamada, Hiromichi Mutoh, Kaoru Umehara, Toshio Miyase, Tsutomu Warashina, Hiroaki Aoshima, Homare Tabata, Kouichi Matsubara, “Matrix Metalloproteinase-2 Inhibitors from Clinopodium chinense var.parviflorum
    J. Nat. Prod. , 72, 1379-1384 (2009)
  • Toshihiro Murata, Toshio Miyase, Tsutomu Warashina, Fumihiko Yoshizaki, “Meehanines A-K, Spermidine Alkaloidal Glycosides from Meehania urticifolia
    J. Nat. Prod. , 72, 1049-1056 (2009)
  • Kyoko Kobayashi, Keiko Yamada, Toshihiro Murata, Tatsuya Hasegawa, Fumihide Takano, Kazutaka Koga, Shinji Fushiya, Javzan Batkhuu, Fumihiko Yoshizaki, “Constituents of Rhodiola rosea Showing Inhibitory Effect on Lipase Activity in Mouse Plasma and Alimentary Canal”
    Planta Med. , 74, 1716-1719 (2008)
  • Toshihiro Murata, Yuka Endo, Toshio Miyase, Fumihiko Yoshizaki, “Iridoid Glycoside Constituents of Stachys lanata
    J. Nat. Prod. 71, 1768-1770 (2008)
  • Kyoko Kobayashi, Satomi Ihara, Ayumi Kobata, Kana Itoh, Nae Kusunoki, Fumihiko Yoshizaki, “Inhibitory Effect of Myrica Bark on Lipase Activity in Mouse Plasma and Gastrointestinal Tract”
    J. Med. Food, 11, 289-293 (2008)
  • Kyoko Kobayashi, Sachi Nagaoka, Sonomi Matsuyama, Fumihiko Yoshizaki, “Investigation of Decoction Procedure of Maoto Described in Shokan-ron Using Mouse Amylase Inhibitory Activity”
    Kampo Med. , 59, 477-482 (2008)
  • Satomi Ihara, Fumihide Takano, Kyoko Kobayashi, Tomoko Ogiwara, Nobuo Yahagi, Remiko Yahagi, Shinji Fushiya, Tomihisa Ohta, Fumihiko Yoshizaki, “Distinction of water-soluble constituents between some Paecilomyces (=Isaria) and Cordyceps fungi by capillary electrophoresis”
    J. Nat. Med. , 60, 243-247 (2006)
  • Kyoko Kobayashi, Tamaki Ishihara, Eriko Khono, Toshio Miyase, Fumihiko Yoshizaki, “Constituents of Stem Bark of Callistemon rigidus Showing Inhibitory Effects on Mouse α-Amylase Activity”
    Biol. Pharm. Bull. , 29, 1275-1277 (2006)
  • Kyoko Kobayashi, Mayumi Kaneko, Fumihiko Yoshizaki, “Estimation of Amount of Total Tannins in Rhubarb and Chinese Medicinal Prescriptions Containing Rhubarb Using Mouse Amylase Inhibition Activity”
    Natural Medicines, 59, 97-103 (2005)
  • Kenroh Sasaki, Naoko Tateoka, Hideyoshi Ando, Fumihiko Yoshizaki, “Effect of flavones on rat brain and lung matrix metalloproteinase activity measured by film in-situ zymography”
    Journal of Pharmacy and Pharmacology, 57, 459-465 (2005)
  • Kyoko Kobayashi, Tomoko Takahashi, Fumihide Takano, Shinji Fushiya, Javzan Batkhuu, Chinbat Sanchir, Fumihiko Yoshizaki, “Survey of the Influence of Mongolian Plants on Lipase Activity in Mouse Plasma and Gastrointestinal Tube”
    Nat. Med. , 58, 204-208 (2004)
  • Kenroh Sasaki, So-ichi Ito, Yusuke Nakamura, Fumihiko Yoshizaki, “Effect of Saiko-ka-ryukotsu-borei-to on Amylase Activity in Mice”
    Biol. Pharm. Bull. , 27, 1688-1690 (2004) Kenroh Sasaki, Hidetomo Abe, Tomoko Honma, Fumihiko Yoshizaki, “Recovery of Oral Candidosis by Oral Ointment Containing Antifungal Naphthoquinone Derivatives Isolated from Shikon”
    Kampo Med. , 55, 261-264 (2004)
  • Kyoko Kobayashi, Eriko Baba, Shinji Fushiya, Fumihide Takano, Javzan Batkhuu, Tzezenjiin Dash, Chinbat Sanchir, Fumihiko Yoshizaki, “Screening of Mongolian Plants for Influence on Amylase Activity in Mouse Plasma and Gastrointestinal Tube”
    Biol. Pharm. Bull. , 26, 1045-1048 (2003)
  • Satomi Ihara, Harumi Shimoda, Yumiko Akiho, Fumihiko Yoshizaki, “Application of Capillary Electrophoresis to Estimate Synephrine Levels in Evodia Fruit”
    Nat. Med. , 57, 110-113 (2003)
  • Kenroh Sasaki, Hidetomo Abe, Fumihiko Yoshizaki, “In Vitro Antifungal Activity of Naphthoquinone Derivatives”
    Biol. Pharm. Bull. , 25, 669-670 (2002)
  • Kenroh Sasaki, Fumihiko Yoshizaki, “Nobiletin as a Tyrosinase Inhibitor from the Peel of CitrusFruit”
    Biol. Pharm. Bull. , 25, 806-808 (2002)

15 Radiopharmacy

Research Projects

  • Yamamoto F., Development and prospects of tumor directional polymer particles as molecular imaging probe for nuclear medicine., J. Tohoku Med. Pharm. Univ., 63, 21-43 (2016)
  • Kuwahara Y., Urushihara Y., Saito Y., Yamamoto Y., Tomita K., Sato T., Yamamoto F., Kurimasa A., Fukumoto M., Ionizing radiation-induced cell death; apotosis, autophagy and necroptosis, J. Tohoku Med. Pharm. Univ., 63, 63-70 (2016)
  • Kuwahara Y., Roudkenar M.H., Suzuki M., Urushihara Y., Fukumoto M., Saito Y., Fukumoto M., The involvement of mitochondrial membrane potential in cross-resistance between radiation and docetaxel, Int. J. Radiat. Oncol. Biol. Phys. 96, 556-565 (2016)
  • Yamamoto Y., Arai J., Hisa T., Saito Y., Mukai T., Ohshima T., Maeda M., and Yamamoto F.、Isomeric iodinated analogs of nimesulide: synthesis, physicochemical characterization, cyclooxygenase-2 inhibitory activity, and transport across Caco-2 cells, Bioorg. Med. Chem. 24, 3727-33 (2016)
  • Kurihara K., Ueda M., Hara I., Hara E., Sano K, Makino A., Ozeki E., Yamamoto F., Saji H., Togashi K., Kimura S., Inflammation-Induced Synergetic Enhancement of Nanoparticle Treatments with DOXIL® and 90 Y-Lactosome for Orthotopic Mammary Tumor, J. Nanopart. Res. 18, 137 (2016)
  • Yamamoto Y., Hisa T., Arai J., Saito Y., Yamamoto F., Mukai T., Ohshima T., Maeda M. and Ohkubo Y., Isomeric Methoxy Analogs of Nimesulide for Development of Brain Cyclooxygense-2 (COX-2) targeted Imaging Agents: Synthesis, in Vitro COX-2-Inhibitory Potency, and Cellular Transport Properties, Bioorg. Med. Chem. 23, 6807-6814 (2015)
  • Sugimoto C., Hasegawa A., Saito Y., Fukuyo Y., Chiu KB., Cai Y., Breed MW., Mori K., Roy CJ., Lackner AA., Kim WK., Didier ES. and Kuroda MJ., Differentiation Kinetics of Blood Monocytes and Dendritic Cells in Macaques: Insights to Understanding Human Myeloid Cell Development., J. Immunol. 195(4), 1774-81 (2015)
  • Saito Y., Abiko R., Kishida A., Kuwahara Y., Yamamoto Y., Yamamoto F., Fukumoto M., and Ohkubo Y., Loss of EGF-dependent cell proliferation ability on radioresistant cell HepG2-8960-R, Cell Biochem. Funct., 33, 73-9 (2015)
  • Yuqin Wang, Tomohiko Fukuda, Tomoya Isaji, Jishun Lu, Wei Gu, Ho-hsun Lee, Yasuhito Ohkubo, Yoshihiro Kamada, Naoyuki Taniguchi, Eiji Miyoshi Jianguo Gu, Loss of α1,6-fucosyltransferase suppressed liver regeneration: implication of core fucose in the regulation of growth factor receptor-mediated cellular signaling, Sci. Rep., 5, 8264 (2015)
  • Fukumoto M., Tatsuya Amanuma T., Yoshikazu Kuwahara Y., Shimura T., Suzuki M., Mori S.,Kumamoto H., Saito Y., Ohkubo Y., Duan Z., Sano K., Oguchi T., Kainuma K., Usami S., Kinoshita K., Lee I. and Fukumoto M., Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance., Cancer Science, 105, 1351-1359 (2014)
  • Hayashi K., Yasuoka Y., Nagahama H., Muto J., Suzuki T., Homma Y., Yamamoto F., Takahashi K., Mukai T., Anomalous change of atmospheric radon concentration before the 2011 Tohoku-Oki Earthquake in Japan, KEK 2014, Proceedings of the 15th Workshop on Environmental Radioactivity., 23-28 (2014)
  • Tanaka R., Seki Y., Saito Y., Kamiya M., Fujita M., Okutsu H., Iyoda T., Takai T., Owaki T., Yajima H., Taira J., Hayashi R., Kodama H., Matsunaga T., and Fukai F., Tenascin-C-derived peptide TNIIIA2 highly enhances cell survival and platelet-derived growth factor (PDGF)-dependent cell proliferation through potentiated and sustained activation of integrin α5β1., J. Biol. Chem., 289, 17699-17708 (2014)
  • Yamamoto F., Kimura M., Makino A., Yamamoto Y., Saito Y., Ozeki E., Kimura S., Ohkubo Y., Synthesis and initial evaluation of radioiodine labeled A3B-type Lactosome as an imaging probe of tumor and/or inflammation., J. Nucl. Med., 55, (Suppl 1), 203P-204P (2014)
  • Kuwahara Y, Mori M, Kitahara S, Fukumoto M, Ezaki T, Mori S, Echigo S, Ohkubo Y, Fukumoto M., Targeting of tumor endothelial cells combining 2 Gy/day of X-ray with Everolimus is the effective modality for overcoming clinically relevant radioresistant tumors., Cancer Med., 3, 310-21 (2014)
  • Hara E., Makino A., Kurihara K., Ueda M., Hara I., Kawabe T., Yamamoto F., Ozeki E., Togashi K., Kimura S., Radionuclide Therapy Using Nanoparticle of 131I-Lactosome in Combination with Percutaneous Ethanol Injection Therapy., J. Nanopart. Res., 15(12), article:2131 (2013)
  • Yamamoto F., Yamahara R., Makino A., Kurihara K., Tsukada H., Hara E., Hara I., Kizaka-Kondoh S., Ohkubo Y., Ozeki E., Kimura S., Radiosynthesis and initial evaluation of 18F labeled nanocarrier composed of poly(L-lactic acid)-block-poly(sarcosine) amphiphilic polydepsipeptide., Nucl. Med. Biol., 40(3), 387-394 (2013)
  • Fujino T., Takeuchi A., Maruko-Ohtake A., Ohtake Y., Satoh J., Kobayashi T., Tanaka T., Ito H., Sakamaki R., Kashimura R., Ando K., Nishimaki-Mogami T., Ohkubo Y., Kitamura N., Sato R., Kikugawa K., Hayakawa M., Critical role of farnesoid X receptor (FXR) for hepatocellular carcinoma cell proliferation., J. Biochem., 152(6), 577-86 (2012)
  • Kobayashi T., Saito Y., Ohtake Y., Maruko A., Yamamoto Y., Yamamoto F., Kuwahara Y., Fukumoto M.,Fukumoto M., Ohkubo Y., Effect of aging on norepinephrine-related proliferative response in primary cultured periportal and perivenous hepatocytes., Am. J. Physiol., 303(7), G861-9 (2012)
  • Hara E., Makino A., Kurihara K., Yamamoto F., Ozeki E., Kimura S., Pharmacokinetic Change of Nanoparticulate Formulation “Lactosome” on Multiple Administrations., Int. Immunopharmacol., 14(3), 261-266 (2012)
  • Makino A., Hara E., Hara I., Yamahara R., Kurihara K., Ozeki E., Yamamoto F., Kimura S., Control of in vivo blood clearance time of polymeric micelle by stereochemistry of amphiphilic polydepsipeptides, J. Control. Release, 161(3), 821-825 (2012)
  • Itagaki K., Naito T., Iwakiri R., Haga M., Miura S., Saito Y., Owaki T., Kamiya S., Iyoda T., Yajima H., Iwashita S., Ejiri SI., Fukai F., Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment, J. Biol. Chem., 287(19), 16037-46 (2012)
  • Kim J., Kino T., Kato H., Yamamoto F., Sano K., Mukai T., Maeda M., 5-O-(4-[125I]Iodobenzyl)-L-ascorbic acid: Electrophilic Radioiodination and Biodistribution in Mice., Chem. Pharm. Bull., 60(2), 235-40 (2012)
  • Makino A., Takeuchi E., Hara I., Yamahara R., Ozeki E., Kurihara K., Yamamoto F., Kimura S., Evasion from the accelerated blood clearance (ABC) phenomenon of "Lactosome" by micelle size control., Mol. Imaging Biol., 14 (Suppl.1), S67 (2012)
  • Kuwahara Y., Oikawa T., Ochiai Y., Rouhkenar MH., Fukumoto M., Shimura T., Ohtake Y., Ohkubo Y., Mori S., Uchiyama Y., Fukumoto M., Enhancement of autophagy is a potential modality for tumors refractory to radiotherapy, Cell Death and Disease 2, e177 (2011)
  • Yamamoto Y., Toyohara J., Ishiwata K., Sano K., Yamamoto F., Mukai T., Maeda M., 11C-Labeled Analogs of Indomethacin Esters and Amides for Brain Cyclooxygenase-2 Imaging: Radiosynthesis, In Vitro Evaluation and In Vivo Characteristics in Mice., Chem. Pharm. Bull. 59(8), 938-946 (2011)
  • Makino A., Yamahara R., Hara I., Takeuchi E., Ozeki E., Kurihara K., Kizaka-Kondoh S., Yamamoto F., Shimizu A., Kimura S., "Lactosome" a potential nano-ordered carrier for molecular imaging composed of a poly(L-lactic acid) - block -poly(sarcosine) amphiphilic polydepsipeptide and poly(lactic acid) derivatives., Mol. Imaging Biol. 12 (Suppl.2), S812 (2010)
  • Takeuchi E., Hara I., Yamahara R., Makino A., Kurihara K., Yamamoto F., Kizaka-Kondoh S., Shimizu A., Ozeki E., Kimura S., ABC phenomenon with lactosome studied by in vivo NIRF imaging., Mol. Imaging Biol. 12 (Suppl.2), S835 (2010)
  • Hara I., Yamahara R., Takeuchi E., Makino A., Kurihara K., Yamamoto F., Kizaka-Kondoh S., Shimizu A., Ozeki E., Kimura S., Development of Near-Infrared Fluorescence Probes for In Vivo Tumor Imaging with Using an Amphiphilic Polydepsipeptide Micelle "Lactosome"., Mol. Imaging Biol. 12 (Suppl.2), S873 (2010)
  • Kurihara K., Yamamoto F., Yamaguchi M., Arakawa Y., Hara I., Ozeki E., Makino A., Togashi K., Shimizu A., Kimura S., A fluorescent imaging of the murine orthotopic brain tumor, using new nanocarrier "Lactosome"., Mol. Imaging Biol. 12 (Suppl.2), S876 (2010)
  • Yamamoto F., Yamahara R., Kurihara K., Takeuchi E., Hara I., Makino A., Kizaka-Kondoh S., Shimizu A., Ozeki E., Kimura S., Development of novel radioiodine labeled nanocarrier "Lactosome" and initial evaluation as a tumor imaging probe., Mol. Imaging Biol. 12 (Suppl.2), S948 (2010)
  • Yamahara R., Yamamoto F., Kurihara K., Tsukada H., Takeuchi E., Hara I., Makino A., Shimizu A., Kizaka-Kondoh S., Ozeki E., Kimura S., Development of a novel radioisotope labeled tumor imaging probe for PET "18F-Lactosome"., Mol. Imaging Biol. 12 (Suppl.2), S995 (2010)
  • Sano Y., Motomura T., Yamamoto F., Fukuda M., Mukai T., Maeda M., 1-(3’-[125I]Iodophenyl)-3-methy-2-pyrazolin-5-one: Preparation, Solution Stability, and Biodistribution in Normal Mice., Chem. Pharm. Bull. 58(8), 1020-1025 (2010)
  • Yamamoto F., Yamahara R., Kurihara K., Tsukada H., Takeuchi E., Hara I., Makino A., Kizaka-Kondoh S., Ozeki E., Kimura S., Development and initial evaluation of novel fluorine-18 labeled nanocarrier "Lactosome" as a tumor imaging probe for PET., J. Nucl. Med., 51(5), (Suppl 2), 304P (2010)
  • Ohtake Y., Kobayashi T., Maruko A., Ohishi N., Yamamoto F., Katoh S., Ohkubo Y., Norepinephrine modulates the zonally different hepatocyte proliferation through the regulation of transglutaminase activity., Am. J. Physiol. 299(1), G106-114 (2010)
  • Kuwahara Y., Mori M., Oikawa T., Shimura T., Ohtake Y., Mori S., Ohkubo Y., Fukumoto M., The modified high-density survival assay is the useful tool to predict the effectiveness of fractionated radiation exposure., J. Radiat. Res. 51, 297-302 (2010)
  • Maruko A., Ohtake Y., Kawaguchi M., Kobayashi T., Baba T., Kuwahara Y., Nakagawa H., Shimura T., Fukumoto M., Ohkubo Y., X-irradiation-induced down-regulation of the EGF receptor in primary cultured rat hepatocytes., Radiat. Res. 173, 620-628 (2010)

16 Biochemistry

Research Projects

  • Cai J, Song B, Cai Y, Ma Y, Lam A, Magiera J, Sekar S, Wyse BD., Ambo A, Sasaki Y, Lazarus LH., Smith MT., Li T, Endomorphin analogues with mixed μ-opioid (MOP) receptor agonism/δ-opioid (DOP) receptor antagonism and lacking β-arrestin2 recruitment activity. Bioorg. Med. Chem. in press (2014)
  • Hosono Y, Abe T, Higuchi M, Kajii K, Sakuraba S, Tada S, Enomoto T, Seki M. Tipin Functions in the Protection against Topoisomerase I Inhibitor. J. Biol. Chem. in press (2014)
  • Hosono Y, Abe T, Ishiai M, Islam MN, Arakawa H, Wang W, Takeda S, Ishii Y, Takata M, Seki M, Enomoto T. Tumor suppressor RecQL5 controls recombination induced by DNA crosslinking agents. Biochim. Biophys. Acta. 1843, 1002-1012 (2014).
  • Nakabayashi Y, Kawashima S, Enomoto T, Seki M, Horikoshi M. Roles of common subunits within distinct multisubunit complexes. Proc. Natl. Acad. Sci. USA. 111, 699-704 (2014).
  • Tamari Y, Nawata H, Inoue E, Yoshimura A, Yoshii H, Kashino G, Seki M, Enomoto T, Watanabe M, Tano K. Protective roles of ascorbic acid in oxidative stress induced by depletion of superoxide dismutase in vertebrate cells. Free Radical Research 47, 1-7 (2013).
  • Ambo A, Ohkatsu H, Minamizawa M, Watanabe H, Sugawara S, Nitta K, Tsuda Y, Okada Y, Sasaki Y.Relationship between structure and P-glycoprotein inhibitory activity of dimeric peptides related to the Dmt-Tic pharmacophore. Bioorg. Med. Chem. Lett. 22, 2192-2194 (2012).
  • Endo H, Nakabayashi Y, Kawashima S, Enomoto T, Seki M, Horikoshi M. Nucleosome surface containing nucleosomal DNA entry/exit site regulates H3-K36me3 via association with RNA polymerase II and Set2. Genes Cells 17, 65-81 (2012).
  • Lai, MS, Seki M, Tada S, Enomoto T. Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1. Biochem. Biophys. Res. Commun. 427, 682-686 (2012).
  • Nomura H, Yoshimura A, Edo T, Kanno SI, Tada S, Seki M, Yasui, A, Enomoto T. WRNIP1 accumulates at laser light irradiated sites rapidly via its ubiquitin-binding zinc finger domain and independently from its ATPase domain. Biochem. Biophys. Res. Commun. 417, 1145-1150 (2012).
  • Saito Y, Ono T, Takeda N, Nohmi T, Seki M, Enomoto T, Noda T, Uehara Y. Embryonic Lethality in Mice Lacking Mismatch-Specific Thymine DNA Glycosylase Is Partially Prevented by DOPS, a Precursor of Noradrenaline. Tohoku J. Exp. Med. 226, 75-83 (2012).
  • Nakamoto K, Nishinaka T, Ambo A, Mankura M, Kasuya F, Tokuyama S. Possible involvement of β-endorphin in docosahexaenoic acid-induced antinociception. Eur. J. Pharmacol. 666, 100-104 (2011).
  • Kawashima S, Nakabayashi Y, Matsubara K, Sano N, Enomoto T, Tanaka K, Seki M, Horikoshi M. Global analysis of core histones reveals nucleosomal surfaces required for chromosome bi-orientation. EMBO J. 30, 3353-3367 (2011).
  • Yoshimura A, Akita M, Hosono Y, Abe T, Kobayashi M, Yamamoto KI, Tada S, Seki M, Enomoto T. Functional relationship between Claspin and Rad17. Biochem. Biophys. Res. Commun. 414, 298-303 (2011).
  • Ishikawa K, Ohsumi T, Tada S, Natsume R, Kundu L-R, Nozaki N, Senda T, Enomoto T, Horikoshi M, Seki M. The roles of histone chaperone CIA/Asf1 in nascent DNA elongation during nucleosome replication. Genes Cells 16, 1050-1062 (2011).
  • Abe T, Sugimura K, Hosono Y, Takami Y, Akita M, Yoshimura A, Tada S, Nakayama T, Murofushi H, Okumura K, Takeda S, Horikoshi M, Seki M, Enomoto T. The histone chaperone FACT maintains normal replication fork rates. J. Biol. Chem. 286, 30504-30512 (2011).
  • Kanamori M, Seki M, Yoshimura A, Tsurimoto T, Tada S, Enomoto T. WRNIP1 promotes binding of WRN to template-primer DNA. Biol. Pharm. Bull. 34, 1314-1318 (2011).
  • Hosono Y, Abe T, Ishiai M, Takata M, Enomoto T, Seki M. The role of SNM1 family nucleases in etoposide-induced apoptosis. Biochem. Biophys. Res. Commun. 410, 568-573 (2011).
  • Abe T, Yoshimura A, Hosono Y, Tada S, Seki M, Enomoto T. The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells. Biochim. Biophys. Acta 1813, 473-479 (2011).
  • Ohkawa M, Ohno Y, Masuko K, Takeuchi A, Suda K, Kubo A, Kawahara R, Okazaki S, Tanaka T, Saya H, Seki M, Enomoto T, Yagi H, Hashimoto Y, Masuko T. Oncogenicity of L-type amino-acid transporter 1 (LAT1) revealed by targeted gene disruption in chicken DT40 cells: LAT1 is a promising molecular target for human cancer therapy. Biochem. Biophys. Res. Commun. 406,649-655 (2011).
  • Kundu LR, Seki M, Watanabe N, Murofushi H, Furukohri A, Waga S, Score AJ, Blow JJ, Horikoshi M, Enomoto T, Tada S. Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication. Biochim. Biophys. Acta. 1813, 1129-1136 (2011).
  • Balboni G, Marzola E, Sasaki Y, Ambo A, Marczak ED, Lazarus LH, Salvadori S. Role of 2′,6′-dimethyl-l-tyrosine (Dmt) in some opioid lead compounds. Bioorg. Med. Chem. 18, 6024-6030 (2010).
  • Inoue E, Tano K, Yoshii H, Nakamura J, Tada S, Watanabe M, Seki M, Enomoto T. SOD1 is essential for the viability of DT40 cells and nuclear SOD1 functions as a guardian of genomic DNA. J. Nucleic Acid Aug5 pil:795946 (2010).
  • Endo H, Kawashima S, Sato L, Lai MS, Enomoto T, Seki M, Horikoshi M. Chromatin dynamics mediated by histone modifiers and histone chaperones in post-replicative recombination. Genes Cells 15, 945-958 (2010).
  • Kundu LR, Kumata Y, Kakusho N, Watanabe S, Furukohri A, Waga S, Seki M, Masai H, Enomoto T, Tada S. Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. Nucleic Acids Res. 38, 5409-5418 (2010).
  • Balboni G, Trapella C, Sasaki Y, Ambo A, Marczak ED, Lazarus LH, Salvadori S. Influence of the side chain next to C-terminal benzimidazole in opioid pseudopeptides containing the Dmt-Tic pharmacophore. J. Med. Chem. 52, 5556-5559 (2009).
  • Sakamoto M, Noguchi S, Kawashima S, Okada Y, Enomoto T, Seki M, Horikoshi M. Global analysis of mutual interaction surfaces of nucleosomes with comprehensive point mutants. Genes Cells 14, 1271-1330 (2009).
  • Yoshimura A, Seki M, Kanamori M, Tateishi S, Tsurimoto T, Tada S, Enomoto T. Physical and functional interaction between WRNIP1 and RAD18. Genes Genet. System 84, 171-178 (2009).
  • Ohuchi T, Seki M, Kugou K, Tada S, Ohta K, Enomoto T. Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication. DNA Repair 8, 690-696 (2009).
  • Takada S, Inoue E, Tano K, Yoshii H, Abe T, Yoshimura A, Akita M, Tada S, Watanabe M, Seki M, Enomoto T. Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2. Biochem. Biophys. Res. Commun. 379, 233-238 (2009).
  • Tsuyama T, Watanabe S, Aoki A, Cho Y, Seki M, Enomoto T, Tada S. Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts. Mol. Biol. Cell 20, 937-947 (2009).
  • Sasaki Y, Minamizawa M, Ambo A, Sugawara S, Ogawa Y, Nitta K. Cell-penetrating peptide-conjugated XIAP-inhibitory cyclic hexapeptides enter into Jurkat cells and inhibit cell proliferation. FEBS J. 275, 6011-6021 (2008).
  • Ryu EK, Wu Z, Chen K, Lazarus LH, Marczak ED, Sasaki Y, Ambo A, Salvadori S, Ren C, Zhao H, Balboni G, Chen X. Synthesis of a potent and selective 18F-labeled delta-opioid receptor antagonist derived from the Dmt-Tic pharmacophore for positron emission tomography imaging. J. Med. Chem. 51, 1817-1823 (2008).
  • Nishino K, Inoue E, Takada S, Abe T, Akita M, Yoshimura A, Tada S, Kobayashi M, Yamamoto K, Seki M, Enomoto T. A novel function for Rad17 in homologous recombination. Genes Genet. System. 83, 427-431 (2008).
  • Abe T, Ishiai M, Hosono Y, Yoshimura A, Tsuji H, Tada S, Adachi N, Koyama H, Takata M, Takeda S, Enomoto T, Seki M. KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation. Cell Signal. 20, 1978-1985 (2008).
  • Ohuchi T, Seki M, Enomoto T. Nuclear localization of Rad52 is pre-requisite for its sumoylation. Biochem. Biophys. Res. Commun. 372, 126-130 (2008).
  • Ohuchi T, Seki M, Branzei D, Maeda D, Ui A, Ogiwara H, Tada S, Enomoto T. Rad52 sumoylation and its involvement in the efficient induction of homologous recombination. DNA Repair 7, 879-889 (2008).
  • Hayashi T, Seki M, Yoshimura A, Inoue E, Kusa Y, Tada S, Enomoto T. Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability. Genes Genet. Syst. 83, 95-100 (2008).
  • Otsuki M, *Seki, M., Inoue, E., Abe. T., Narita, Y., Yoshimura, A., Tada, S., Ishii, Y., and Enomoto, T. Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIα. Biochim. Biophys. Acta 1782, 75-81 (2008).
  • Ambo A, Kohara H, Kawano S, Sasaki Y. Opioid receptor-like 1 (ORL1) receptor binding and the biological properties of Ac-Arg-Tyr-Tyr-Arg-Ile-Arg-NH2 and its analogs. J. Pept. Sci. 13, 672-678 (2007).
  • Kawano S, Ito R, Nishiyama M, Kubo M, Matsushima T, Minamisawa M, Ambo A, Sasaki Y. Receptor Binding Properties and Antinociceptive Effects of Chimeric Peptides Consisting of a μ-Opioid Receptor Agonist and an ORL1 Receptor Antagonist. Biol. Pharm. Bull. 30, 1260-1264 (2007).
  • Li T, Shiotani K, Miyazaki A, Tsuda Y, Ambo A, Sasaki Y, Jinsmaa Y, Marczak E, Bryant SD, Lazarus LH, Okada Y. Bifunctional [2′,6′-Dimethyl-l-tyrosine1]endomorphin-2 Analogues Substituted at Position 3 with Alkylated Phenylalanine Derivatives Yield Potent Mixed μ-Agonist/δ-Antagonist and Dual μ-Agonist/δ-Agonist Opioid Ligands. J. Med. Chem. 50, 2753-2766 (2007).
  • Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T. Functional interactions between BLM and XRCC3 in the cell. J. Cell Biol. 179, 53-63 (2007).
  • Ogiwara H, Ohuchi T, Ui A, Tada S, Enomoto T, Seki M. Ctf18 is required for homologous recombination-mediated double strand break repair. Nuc. Acids Res. 35, 4989-5000 (2007).
  • Lai MS, Seki M, Ui A, Enomoto T. Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion. EMBO Reports 8, 685-690 (2007).
  • Dong YP, Seki M, Yoshimura A, Inoue E, Furukawa S, Tada S, Enomoto T. WRN functions in a RAD18-dependent damage avoidance pathway. Biol. Pharm. Bull. 30, 1080-1083 (2007).
  • Ui A, Seki M, Ogiwara H, Lai MS, Yamamoto K, Tada S, Enomoto T. Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells. Biochem. Biophys. Res. Commun. 356, 1031-1037 (2007).
  • Ogiwara H, Enomoto T, Seki M. INO80 chromatin remodeling complex functions in sister chromatid cohesion. Cell Cycle 6, 1090-1095 (2007).
  • Ogiwara H, Ui A, Kawashima S, Kugou K, Onoda F, Iwahashi H, Harata M, Ohta K, Enomoto T, Seki M. Actin-related protein Arp4 functions in kinetochore construction. Nuc. Acids Res. 35, 3109-3117 (2007).
  • Kawashima S, Ogiwara H, Tada S, Harata M, Wintersberger U, Enomoto T, Seki M. INO80 complex is required for damage-induced recombination. Biochem. Biophys. Res. Commun. 355, 835-841 (2007).
  • Otsuki M, Seki M, Kawabe Y, Inoue E, Peng DY, Abe T, Kato G, Yoshimura A, Tada S, Enomoto T. WRN counteracts the NHEJ pathway upon camptothecin exposure. Biochem. Biophys. Res. Commun. 355, 477-482 (2007).
  • Tomizawa Y, Ui A, Onoda F, Ogiwara H, Tada S, Enomoto T, and Seki M. Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells. Genes Genet. Syst. 82, 157-160 (2007).
  • Kumata Y, Tada S, Yamanada Y, Tsuyama T, Kobayashi T, Dong YP, Ikegami K, Murofushi H, Seki M, Enomoto T. Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts. Biochim. Biophys. Acta 1773, 556-564 (2007).
  • Ogiwara H, Ui A, Lai MS, Enomoto T, Seki M. Chl1 and Ctf4 are required for damage-induced recombinations. Biochem. Biophys. Res. Commun. 354, 222-226 (2007).
  • Ogiwara H, Ui A, Enomoto T, Seki M. Role of Elg1 protein in the double-strand breaks repair. Nuc. Acids Res. 35, 353-362 (2007).
  • Sasaki Y, Kawano S, Kohara H, Watanabe H, Ambo A. ORL1 and opioid receptor preferences of nociceptin and dynorphin A analogues with Dmp substituted for N-terminal aromatic residues Bioorg. Med. Chem. 14, 2433-2437 (2006).
  • Kawano S, Ambo A, Sasaki Y. Synthesis and receptor binding properties of chimeric peptides containing a μ-opioid receptor ligand and nociceptin/orphanin FQ receptor ligand Ac-RYYRIK-amide Bioorg. Med. Chem. Lett. 16, 4839-4841 (2006).
  • Balboni G, Onnis V, Congiu C, Zotti M, Sasaki Y, Ambo A, Bryant SD, Jinsmaa Y, Lazarus LH, Trapella C, Salvadori S. Effect of lysine at C-terminus of the Dmt-Tic opioid pharmacophore J. Med. Chem. 49, 5610-5617 (2006).
  • Tsuyama T, Inou K, Seki M, Seki T, Kumata Y, Kobayashi T, Kimura K, Hanaoka F, Enomoto T, Tada S. Chromatin loading of Smc5/6 is induced by DNA replication but not by DNA double-strand breaks. Biochem. Biophys. Res. Commun. 351, 935-939 (2006).
  • Yoshimura A, Seki M, Hayashi T, Kusa Y, Tada S, Ishii Y, Enomoto T. Functional Relationships between Rad18 and WRNIP1 in vertebrate cells. Biol. Pharm. Bull. 29, 2192-2196 (2006).
  • Yoshimura A, Nishino K, Takezawa J, Tada S, Kobayashi T, Sonoda E, Kawamoto T, Takeda S, Ishii Y, Yamada K, Enomoto T, Seki M. A novel Rad18 function involved in the protection of the vertebrate genome after exposure to camptothecin. DNA Repair 5, 1307-1316 (2006).
  • Branzei D, Sollier J, Liberi G, Zhao X, Maeda D, Seki M, Enomoto, T, Ohta K, Foiani M. Ubc9 and Mms21 mediated sumoylation counteracts recombinogenic events at damaged replication forks. Cell 127, 509-522 (2006).
  • Karmakar P, Seki M, Kanamori M, Hashiguchi K, Ohtsuki M, Murata E, Inoue E, Tada S, Lan L, Yasui A, Enomoto T. BLM is an early responder to DNA double-strand breaks. Biochem. Biophys. Res. Commun. 348, 62-69 (2006).
  • Seki M, Nakagawa T, Seki T, Kato G, Tada T, Takahashi Y, Yoshimura A, Kobayashi T, Aoki A, Otsuki M, Habermann FA, Tanabe H, Ishii Y, Enomoto T. Bloom helicase and DNA topoisomerase IIIα are involved in the dissolution of sister chromatids. Mol. Cell. Biol. 26, 6299-6307 (2006).
  • Ogiwara H, Ui A, Onoda F, Tada S, Enomoto T, Seki M. Dpb11, the budding yeast homologue of TopBP1, functions with the checkpoint clamp in recombination repair. Nuc. Acids Res. 34, 3389-3398 (2006).
  • Kawabe Y, Seki M, Yoshimura A, Nishino K, Hayashi T, Takeuchi T, Iguchi S, Kusa Y, Ohtsuki M, Tsuyama T, Imamura O, Matsumoto M, Furuichi Y, Tada S, Enomoto T. Analyses of the interaction of WRNIP1 with Werner syndrome protein (WRN) in vitro and in the cell. DNA Repair 5, 816-828 (2006).
  • Li T, Fujita Y, Tsuda Y, Miyazaki A, Ambo A, Sasaki Y, Jinsmaa Y, Bryant SD, Lazarus LH, Okada Y. Development of potent μ-opioid receptor ligands using unique tyrosine analogues of endomorphin-2 J. Med. Chem. 48, 586-592 (2005)
  • Jinsmaa Y, Fujita Y, Shiotani K, Miyazaki A, Li T, Tsuda Y, Okada Y, Ambo A, Sasaki Y, Bryant SD, Lazarus LH. Differentiation of opioid receptor preference by [Dmt1]endomorphin-2-mediated antinociception in the mouse Eur. J. Pharmacol. 509, 37-42 (2005).
  • Li T, Fujita Y, Shiotani K, Miyazaki A, Tsuda Y, Ambo A, Sasaki Y, Jinsmaa Y, Marczak E, Bryant SD, Salvadori S, Lazarus LH, Okada Y. Potent Dmt-Tic pharmacophoric δ- and μ-opioid receptor antagonists J. Med. Chem. 48, 8035-8044 (2005).
  • Tsuyama T, Tada S, Watanabe S, Seki M, Enomoto T. Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts. Nuc. Acids Res. 33, 765-775 (2005).
  • Hirano S, Yamamoto K, Ishiai M, Yamazoe M, Seki M, Matsushita N, Ohzeki M, Yamashita YM, Arakawa H, Buerstedde JM, Enomoto T, Takeda S, Thompson LH, Takata M. Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLM. EMBO J. 24, 418-427 (2005).
  • LeRoy G, Carroll R, Kyin S, Seki M, Cole MD. Identification of RecQL1 as a Holliday junction processing enzyme in human cell lines. Nucleic Acids Res. 33, 6251-6257 (2005).
  • Tsurimoto T, Shinozaki A, Yano M, Seki M, Enomoto T. Human Werner helicase interacting protein 1 (WRNIP1) functions as a novel modulator for DNA polymerase δ. Genes Cells 10, 13-22 (2005).
  • Ui A, Seki M, Ogiwara H, Onodera R, Fukushige S, Onoda F, Enomoto T. The ability of Sgs1 to interact with DNA topoisomerase III is essential for damage-induced recombination. DNA Repair 4, 191-201 (2005).

17 Medical and Pharmaceutical Information Science

Research Projects

  • Aoki,S.; Satoh,K.; Hoshi,K.; Kawakami,J.; Sato,K.; Saito,Y.; Mori,K.; Yoshida,K. “New Method for Aiding Detection of Abnormal Thyroid Function in Patients Making Use of Set of Routine Tests – Applying It in Screening During General Health Check-ups”
    Ningen Dock(Official Journal of Japan Society of Ningen Dock). 2011, 26, 9-16.
  • Aoki,S.; Hoshi,K.; Kawakami,J.; Sato,K.; Satoh,K.; Mori,K.; Sugawara,A.; Saito,Y.; Yoshida, K. “Assisting the diagnosis of Graves’ hyperthyroidism with pattern recognition methods and a set of three routine tests parameters, and their correlations with free T4 levels: Extension to male patients”
    Biomed. Pharmacother. 2011, 65, 95-104.
  • Sato,W.; Hoshi,K.; Kawakami,J.; Sato,K.; Sugawara,A.; Saito,Y.; Yoshida,K. “Assisting the diagnosis of Graves’ hyperthyroidism with Bayesian- type and SOM-type neural networks by making use of a set of three routine tests and their correlation with free T4”
    Biomed. Pharmacother. 2010, 64, 7-15.
  • Sato,K.; Kawakami,J.; Iwaya,K. “Visualization of information on side effects using self-organizing maps (I) - Usefulness of visual information and possibility of predicting side effects - (in Japanese)”
    Yakkyoku. 2010, 61, 324-330.
  • Sato,K.; Kawakami,J.; Iwaya,K; Hayashi,S. “Visualization of the information on side effects using self-organizing maps-Clinical application and its problems-(in Jananese)”
    Yakkyoku. 2010, 61, 475-482.
  • Iwaya,K.; Sato,K.; Kawakami,J. “Visualization and analysis of the information on side effects for hypotensive drugs using self-organizing maps (in Japanese)”
    Medicine and Drug J. 2010, 46, 1730-1732, 1855-1863.
  • Tozuka,K.; Hamada,K.; Sato,K.; Kawakami,J.; Hoshi,K.; Aoki,S. “Data-book for MIC on antibacterial drugs using visualized circle map representation (in Japanese) 2nd edition”
    Jiho. 2010.
  • Aoki,S.; Sato,K.; Hoshi,K.; Kawakami,J.; Mori,K.; Sugawara,A.; Saito,Y.; Yoshida, K. “Applying Support Vector Machine in the Analysis of Medical Data”
    J. Tohoku Pharmaceutical University. 2009, 56, 67-74.
  • Kanazawa,Y.; Kawakami,J.; Hoshi,K.; Kawamura,M.; Iwaya,K.; Sato,W.; Hamada,K.; Sato,K. “A visualization and analysis with the Self-Organizing Map(SOM) for the information of the adverse reaction for antimicrobial agents”
    Jpn. J. Drug Inform. 2007, 9, 124-130.
  • Hoshi,K.; Kawakami,J.; Sato,W.; Sato,K.; Sugawara,A.; Saito,Y.; Yoshida,K. “Assisting the Diagnosis of Thyroid Diseases with Bayesian-Type and SOM-Type Neural Networks Making Use of Routine Data”
    Chem.Pharm.Bull. 2006, 54, 1162-1169.
  • Hoshi,K.; Kawakami,J.; Kumagai,M.; Kasahara,S.; Nishimura,N.; Nakamura,H.; Sato,K. “An Analysis of Thyroid Function Diagnosis Using Bayesian-Type and SOM-Type Neural Networks”
    Chem.Pharm.Bull. 2005, 53, 1570-1574.
  • Kawakami,J.; Hoshi,K.; Ishiyama,A.; Miyagishima,S.; Sato,K.; “Application of a Self-Organizing Map to Quantitative Structure-Activity Relationship Analysis of Carboquinone and Benzodiazepine”
    Chem.Pharm.Bull. 2004, 52, 751-755.
  • Sato,K. “Neural Network Approach to improve the Prediction Ability -Quantitative Structure-Activity Relationships and Classification Problems”
    J.Tohoku Pharmaceutical University. 2003, 50, 41-61.

18 Pharmaceutical Physical Chemistry

Research Projects

  • Noji, I.; Oda, A.; Kobayashi, K.; Takahashi, O. “Computational investigation of the substrate recognition mechanism of protein D-aspartyl (L-isoaspartyl) O-methyltransferase by docking and molecular dynamics simulation studies and application to interpret size exclusion chromatography data”
    J. Chromatogr. B 2011, in press.
  • Oda, A.; Kawakami, T; Kitagawa, Y.; Okumura, M.; Takahashi, O. “Continuous optimization methods for ground-state searches of spin clusters”
    Polyhedron, 2011, in press.
  • Ohta, K.; Goto, T.; Fujii, S.; Kawahata, M.; Oda, A.; Ohta, S.; Yamaguchi, K.; Hirono, S.; Endo, Y. “Crystal structure, docking study and structure-activity relationship of carborane-containing androgen receptor antagonist 3-(12-hydroxymethyl-1,12-dicarba-closo-dodecaboran-1-yl)benzonitrile”
    Bioorg. Med. Chem. 2011, 19, 3540-3548.
  • Sakurai, J.; Kikuchi, T.; Takahashi, O.; Watanabe, K.; Katoh, T. “Enantioselective total synthesis of (+)-stachyflin: A potential anti-influenza A virus agent isolated from a microorganism
    Eur. J. Org. Chem. 2011, 16, 2948-2957.
  • Oda, A.; Kobayashi, K.; Takahashi, O. “Computational study of the three-dimensional structure of N-acetyltransferase 2-acetyl coenzyme A complex”
    Biol. Pharm. Bull. 2010, 33, 1639-1643.
  • Oda, A.; Takahashi, O. “Parameter determination for the charge equilibration method including third- and fourth-order terms applied to non-metallic compounds”
    Chem. Phys. Lett. 2010, 495, 155-159.
  • Takahashi, O.; Kobayashi, K.; Oda, A. “Modeling the enolization of succinimide derivatives, a key step of racemization of aspartic acid residues: Importance of a two-H2O mechanism”
    Chem. Biodiversity 2010, 7, 1349-1356.
  • Oda, A.; Kobayashi, K.; Takahashi, O. “Molecular-dynamics simulations for amyloid β1-42monomer with D-aspartic acid residues using continuous solvent”
    Chem. Biodiversity 2010, 7, 1357-1363.
  • Takahashi, O.; Kobayashi, K.; Oda, A. “Computational insight into the mechanism of serine residue racemization”
    Chem. Biodiversity 2010, 7, 1625-1629.
  • Takahashi, O.; Kobayashi, K.; Oda, A. “computational modeling of the enolization in a direct mechanism of racemization of the aspartic acid residue”
    Chem. Biodiversity 2010, 7, 1630-1633.
  • Kapojos, M. M.; Lee, J.-S.; Oda, T.; Nakazawa, T.; Takahashi, O.; Ukai, K.; Mangindaan, R. E. P.; Rotinsulu, H.; Wewengkang, D. S.; Tsukamoto, S.; Kobayashi, H.; Namikoshi, M. “Two unprecedented cembrene-type terpenes from an Indonesian soft coral Sarcophyton sp. “
    Tetrahedron, 2010, 66, 641-645.
  • Wang, W.; Takahashi, O.; Oda, T.; Nakazawa, T.; Ukai, K.; Mangindaan, R. E. P.; Rotinsulu, H.; Wewengkang, D. S.; Kobayashi, H.;, Tsukamoto, S.; Namikoshi, M. “Lissoclibadins 8-14, polysulfur dopamine-derived alkaloids from the colonial ascidian Lissoclinum cf. badium
    Tetrahedron, 2009, 65, 9598-9603.
  • Oda, A.; Yamaotsu, N.; Hirono, S. “Evaluation of the searching abilities of HBOP and HBSITE for binding pocket detection”
    J. Comput. Chem. 2009, 30, 2728-2737.
  • Oda, A.; Takahashi, O. “Validation of ArgusLab efficiencies for binding free energy calculations”
    Chem-Bio Info. J. 2009, 9, 52-61.
  • Oda, A; Yamaotsu, N.; Hirono, S.; Takahashi, O. “Brownian dynamics simulations of a wild type and mutants of bovine pancreatic trypsin inhibitors”
    Biol. Pharm. Bull. 2008, 31, 2182-2186.
  • Yamaotsu, N.; Oda, A.; Hirono, S. “Determination of ligand-binding sites on proteins using long-range hydrophobic potential”
    Biol. Pharm. Bull. 2008, 31, 1552-1558.
  • Oda, A.; Okayasu, M.; Kamiyama, Y.; Yoshida, T.; Takahashi, O.; Matsuzaki, H. “Evaluation of docking accuracy and investigations of roles of parameters and each term in scoring functions for protein-ligand docking using ArgusLab software”
    Bull. Chem. Soc. Jpn. 2007, 80, 1920-1925.
  • Oda, A.; Tsuchida, K.; Takakura, T.; Yamaotsu, N.; Hirono, S. “Comparison of consensus scoring strategies for evaluating computational models of protein-ligand complexes”
    J. Chem. Inf. Model. 2006, 46, 380-391.
  • Fujita, R.; Yoshisuji, T.; Wakayanagi, S.; Wakamatsu, H.; Matsuzaki, H. “Synthesis of 5(6H)-phenanthridones using Diels-Alder reaction of 3-nitro-2(1H)-quinolones acting as dienophiles”
    Chem. Pharm. Bull. 2006, 54, 204-208.
  • Fujita, R.; Wakayanagi, S.; Wakamatsu, H; Matsuzaki, H. “Novel Diels-Alder reaction of 4-nitro-1(2H)-isoquinolones”
    Chem. Pharm. Bull. 2006, 54, 209-212.

19 Clinical Pharmaceutics

Research Projects

  • Katsuyama,S.; Sasaki,M.; Yagi,T.; Kishikawa,Y.; Nakamura,H. “A Survey of Peripheral Neuropathy Associated with Cancer Chemotherapy”
    J.Jpn.Soc.Hosp.Pharm. 2011,47, 207-210.
  • Kishikawa,Y.; Iwasaki,T.; Ito,M.; Ishikura,K.; Ikeda,K.; Sato,K.; Konno,Y.; Nakamura,H.; Mizugaki,M. ” Comparative Clinical Evaluation of Efficiency and Safety between Original Drug and Generic Products (I) “
    Jpn.J.Generic Med. 2009,3,127-135.
  • Sakuyama,K.; Sasaki,T.; Ujiie,S.; Obata,K.; Mizugaki,M.; Ishikawa,M.; Hiratsuka,M. ” Functional characterization of 17 CYP2D6 allelic variants (CYP2D6.2, 10, 14A-B, 18, 27, 36, 39, 47-51, 53-55, and 57)”
    Drug Metab.Dispos. 2008,36, 2460-2467.
  • Sasaki,T.; Horikawa,M.; Orikasa,K.; Sato,M.; Arai,Y.; Mitachi,Y.; Mizugaki,M.; Ishikawa,M.; Hiratsuka,M. “Possible relationship between the risk of Japanese bladder cancer cases and the CYP4B1 genotype “
    Jpn.J.Clin.Oncol. 2008,38, 634-640.
  • Ujiie,S.; Sasaki,T.; Mizugaki,M.; Ishikawa,M.; Hiratsuka,M. “Functional characterization of 23 allelic variants of thiopurine S-methyltransferase gene (TPMT*2 – *24) “
    Pharmacogenet.Genom. 2008,18, 887-893.
  • Nusuetrong,P.; Pengsuparp,T.; Meksuriyen,D.; Tanitsu,M.; Kikuchi,H.; Mizugaki,M.; Shimazu,K.; Oshima,Y.; Nakahat,N.; Yoshida,M. “Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells “
    Biol. Pharm. Bull. 2008,31, 1115-1120.
  • Kudo,M.; Moteki,T.; Sasaki,T.; Konno,Y.; Ujiie,S.; Onose,A.; Mizugaki,M.; Ishikawa,M.; Hiratsuka,M. “Functional characterization of human xanthine oxidase allelic variants “
    Pharmacogenet.Genom. 2008,18, 243-251.
  • Hanzawa,Y.; Sasaki,T.; Mizugaki,M.; Ishikawa,M.; Hiratsuka,M. “Genetic polymorphisms and haplotype structures of the human CYP2W1 gene in a Japanese population “
    Drug Metab.Dispos. 2008,36, 349-352.
  • Hiratsuka,M.; Hinai,Y.; Sasaki,T.; Konno,Y.; Imagawa,K.; Ishikawa,M.; Mizugaki,M. ” Characterization of human cytochrome p450 enzymes involved in the metabolism of cilostazol “
    Drug Metab.Dispos. 2007,35, 1730-1732.
  • Itoh,K.; Masubuchi,A.; Sasaki,T.; Adachi,M.; Watanabe,N.; Nagata,K.; Yamazoe,Y.; Hiratsuka,M.; Mizugaki,M.; Tanaka,Y. “Genetic polymorphism of aldehyde oxidase in Donryu rats “
    Drug Metab.Dispos. 2007,35, 734-739.
  • Hanzawa,Y.; Sasaki,T.; Hiratsuka,M.; Ishikawa,M.; Mizugaki,M. “Three novel single nucleotide polymorphisms (SNPs) of CYP2S1 gene in Japanese individuals “
    Drug Metab.Pharmacokin . 2007,22, 136-140.
  • Nakagawa,N.; Narahara,K.; Enomoto,E.; Furudate,H.; Yabuta,F.; Tasaka,H.; Inooka,K.; Zenda,A.; Hisamichi,K.; Kishikawa,Y.; Nakamura,H.; Nakamura,H.; Murai,Y.; Hishinuma,T.; Goto,J. “Pharmacotherapy Interventions by Pharmacists Enhance Medication Safety for Ophthalmology Patients -Ophthalmologists Improve Prescribing after Drug Therapy Problems Identified-“
    Jpn.J.Pharm.Health Care Sci.2006,32,747-753
  • Sasaki,T.; Masubuchi,A.; Yamamura,M.; Watanabe,N.; Hiratsuka,M.; Mizugaki,M.; Itoh,K.; Tanaka,Y. ” Rat strain differences in stereospecific 2-oxidation of RS-8359, a reversible and selective MAO-A inhibitor, by aldehyde oxidase “
    Biopharm. Drug Dispos. 2006,27, 247-255.
  • Hiratsuka,M.; Nozawa,H.; Katsumoto,Y.; Moteki,T. ; Sasaki,T. ; Konno,Y.; Mizugaki,M. ” Genetic polymorphisms and haplotype structures of the CYP4A22 gene in a Japanese population “
    Mutat. Res. Fundam. Mol. Mech. Mutagen.2006, 599, 98-104.
  • Hiratsuka,M.; Ebisawa,A.; Sakuyama,K.; Matsubara,Y.; Kure,S.; Soya,Y.; Konno,Y.; Sasaki,T.; Kishiba,A.; Mizugaki,M. ” Competitive allele-specific short oligonucleotide hybridization (CASSOH) with enzyme-linked immunosorbent assay (ELISA) for the detection of pharmacogenetic single nucleotide polymorphisms (SNPs)”
    J. Biochem. Biophys. Methods. 2006,67, 87-94.
  • Sasaki,T.; Goto,E.; Konno,Y. ; Hiratsuka,M. ; Mizugaki,M. ” Three novel single nucleotide polymorphisms of the human thiopurine S-methyltransferase gene in Japanese Individuals “
    Drug Metab. Pharmacokin. 2006,21, 332-336.
  • Tomioka,Y.; Kisara,S.; Yoshizawa,S.; Ozawa,M.; Suzuki,N.; Yamaguchi,H.; Hishinuma,T.; Mizugaki,M.; Goto,J. “Preparation of neocarzinostatin apoprotein mutants and the randomized library on the chromophore-binding cavity “
    Biol.Pharm.Bull. 2006,29, 1010-1014.
  • Yamaguchi,H.; Hishinuma,T.; Endo,N.; Tsukamoto,H.; Kishikawa,Y.; Sato,M.; Murai,Y.; Hiratsuka,M.; Ito,K.; Okamura,C.; Yaegashi,N.; Suzuki,N.; Tomioka,Y.; Goto,J. ” Genetic variation in ABCB1 influences paclitaxel pharmacokinetics in Japanese patients with ovarian cancer “
    Int.J. Gynecol.Cancer.2006,16,979-985.
  • Hiratsuka,M.; Sasaki,T.; Mizugaki,M. ” Genetic testing for pharmacogenetics and its clinical application in drug therapy “
    Clinica.Chimica.Acta.2006,363,177-186.
  • Mizuno,T.; Aoki,M.; Shimada,Y.; Inoue,M.; Nakaya,K. Takahashi,T.; Itoyama,Y.; Kanazawa,M.; Utsumi,A.; Endo,Y.; Nomura,T.; Hiratsuka,M.; Mizugaki,M.; Goto,J.; Hongo,M.; Fukudo,S. ” Gender difference in association between polymorphism of serotonin transporter gene regulatory region and anxiety “
    J.Psychosom.Res.2006,60,91-97.

20 Drug Metabolism and Pharmacokinetics

Research Projects

  • A. Iida, et al. Changes of absorptive and secretory transporting system of (1→3) β-D-glucan based on efflux transporter in indomethacin-induced inflammation. Eur. J. Drug Metab. Pharmacokinet., accepted. 2013.
  • Y. Takizawa, et al. Effects of pharmaceutical excipients on membrane permeability in rat small intestine. Int. J. Pharm., 2013, 453:363-70
  • Y. Takizawa, et al. Characteristics of reversible absorption-enhancing effect of sodium nitroprusside in rat small intestine. Eur. J. Pharm. Sci., 2013, 49:664-70
  • Y. Takizawa, et al. Changes in absorption and excretion of rhodamine123 by sodium nitroprusside. Int. J. Pharm., 2013, 450:31-35
  • K. Yano, et al. Contribution of radixin to P-glycoprotein expression and transport activity in mouse small intestine in vivo. J. Pharm. Sci., 2013, 102:2875-81
  • Y. Idota, et al. Alginate enhances excretion and reduces absorption of strontium and cesium in rats. Biol. Pharm. Bull., 2013, 36:485-91
  • S. Wada, et al. Role of inter-segmental difference in P-glycoprotein expression and activity along rat small intestine in causing double peak phenomena of substrate plasma concentration. Drug Metab. Pharmacokinet., 2013, 28:98-103
  • M. Tomita, et al. Increases in bioavailability of poorly absorbed drug by acylcarnitine application into intestinal lumen in rats. J. Pharm. Sci., 2012, 101:3511-17
  • M. Tomita, et al.. Suppression of efflux transporters in the intestine of endotoxin-treated rats. Int. J. Pharm., 2012, 428:33-38.
  • Y. Takizawa, et al. Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion. Int .J. Pharam., 2012, 426:82-89.
  • K. Morimoto, et al. Developmental changes of brain distribution and localization of oseltamivir and its active metabolite Ro 64-0802 in rats. J. Toxicol. Sci., 2012, 37:1217-23
  • S. Kobayashi, et al. Transport mechanism of flavanone aglycones across Caco-2 cell monolayers and artificial PAMPA membranes. J. Pharm. Pharmacol., 2012, 61:52-60
  • M. Tomita, et al. Nonlinear intestinal absorption of fluorescein isothiocyanate dextran 4,000 caused by absorptive and secretory transporting system. Pharmacol. Pharm., 2011, 2:173-79
  • Y. Takizawa, et al. Effect of aminoguanidine on ischemia/reperfusion injury in rat small intestine. Biol. Pharm. Bull., 2011, 34:1737-43
  • Y. Takizawa, et al. Changes in the localization of ileal P-glycoprotein induced by intestinal ischemia/reperfusion. Biol. Pharm. Bull., 2011, 34:408-14
  • Y. Takizawa, et al. Effects of antioxidants on the ischemia/reperfusion injury in rat. Eur. J. Drug Metab. Pharmacokinet., 2011, 35: 89-95
  • Y. Takizawa, et al. Effects of nitric oxide on mucosal barrier dysfunction during early phase of intestinal ischemia/reperfusion. Eur. J. Pharm. Sci., 2011, 42:246-52
  • N. Doi, et al. Absorption enhancement of acylcarnitine through changes in tight junction protein in Caco-2 Cell monolayers. Drug Metab. Pharmacokinet., 2011, 26:162-70
  • M. Tomita, et al. Changes in the pharmacokinetics of rhodamine123 and bromosulfophtalein in rats treated with lipopolysaccharide. Organ Biol., 2011, 18:145-50
  • T. Kano, et al. Effect of knockdown of ezrin, radixin, and moesin on P-glycoprotein function in HepG2 cells. J. Pharm. Sci., 2011, 100:5308-14
  • K. Morimoto, et al. Effect of milk on the pharmacokinetics of oseltamivir in healthy volunteers. J. Pharm. Sci., 2011, 100:3854-61
  • K. Chiba, et al. Genetic Marker of Statin-induced Rhabdomyolysis. YAKUGAKU ZASSHI, 2011, 131:247-53
  • T. Kano, et al. Enhancement of drug solubility and absorption by copolymers of 2-methacryloyloxyethyl phosphorylcholine and n-butyl methacrylate. Drug Metab. Pharmakinet.,2011, 26:79-86
  • Kensuke Fukiya; Kunio Itoh; Satoshi Yamaguchi; Akiko Kishiba; Mayuko Adachi; Nobuaki Watanabea; Yorihisa Tanaka (Drug metabolism and Pharmacokinetics Research Laboratories,Daiichi-Sankyo Co.,Ltd.a) “A single amino acid substitution confers high cinchonidine oxidation activity comparable with that of rabbit to monkey aldehyde oxidase 1”
    Drug Metab.Dispos. ,2010, 38, 302-307.
  • Yorihisa Tanaka; Kunio Itoh “Properties of Aldehyde Oxidase as a Drug-metabolizing Enzyme”
    Journal of Tohoku Pharmaceutical University. 2009, 56, 1-14.
  • Kunio Itoha; Sayaka Kanedaa; Satoshi Yamaguchia; Hideko Suzukia; Yuko Ichiharaa; Ryo Muratab; Yorihisa Tanakaa “Increased Hepatic but Decreased Adipocytic 11β-Hydroxysteroid Dehydrogenase Type 1 in Type 2 Diabetes Model Goto-Kakizaki Rats”
    Journal of Tohoku Pharmaceutical University. 2009, 56, 81-87.
  • Kunio Itoh; Tasuku Asakawa; Kouichi Hoshino; Mayuko Adachi; Kensuke Fukiya; Nobuaki Watanabea; Yorihisa Tanaka (Drug metabolism and Pharmacokinetics Research Laboratories,Daiichi-Sankyo Co.,Ltd. a) “Functional analysis of aldehyde oxidase using expressed chimeric enzyme between monkey and rat”
    Biol. Pharm. Bull. 2009, 32, 31-35.
  • Kunio Itoh; Mayuko Adachi; Jun Sato; Kanako Shouji; Kensuke Fukiya; Keiko Fujii; Yorihisa Tanaka “Effects of selenium deficiency on aldehyde oxidase 1 in rats”
    Biol. Pharm. Bull. 2009, 32, 190-194.
  • Kunio Itoh “Individual and strain difference of aldehyde oxidase in rat”
    Yakugaku Zasshi. 2009, 129, 1487-1493.
  • Mayuko Adachi; Kunio Itoh; Hikaru Abe ; Yorihisa Tanaka “Heredity Mode of Genetic Polymorphism in Aldehyde Oxidase Activity in Donryu Strain Rats”
    Xenobiotica. 2008, 38, 98-105.
  • Kunio Itoh; Kohei Yamamoto; Mayuko Adachi; Tosiyuki Kosakaa; Yorihisa Tanaka (Core Technology Research Laboratories, Daiichi-sankyo Co.Ltd. a) “Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin△13-reductase (LTB4 12-HD/PGR) responsible for reduction of a double bond of the α,β-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670”
    Xenobiotica. 2008, 38, 249-263.
  • Tasuku Asakawa; Kunio Itoh; Mayuko Adachi; Kouichi Hoshino; Nobuaki Watanabea; Yorihisa Tanaka (Drug metabolism and Pharmacokinetics Research Laboratories,Daiichi-Sankyo Co.,Ltd. a) “Properties of 130 kDa subunit of monkey aldehyde oxidase”
    Biol. Pharm. Bull. 2008, 31, 380-385.
  • Takayoshi Nishiyaa; Michiyuki Katoa; Takami Suzukia; Chikako Marua; Hiroko Kataokaa; Chiharu Hattoria; Kazuhiro Moria; Toshimasa Jindoa; Yorihisa Tanaka; Sunao Manabea (Medicinal Safety Research Laboratories, Daiichi-Sankyo Co.,Ltd. a) “Involvement of cytochrome P450-mediated metabolism in tienilic acid hepatotoxicity in rats”
    Toxicol. Lett. 2008, 183, 81-89.
  • Keiko Fujii; Mika Saito; Koutaro Kato; Yukisumi Iizuka; Kunio Itoh; Yorihisa Tanaka “Effect of selenium deficiency on rat liver drug-metabolizing enzymes”
    Journal of Tohoku Phamaceutical University. 2006, 53, 99-108.
  • Hideaki Niwata; Kaori Maruyama; Fumiko Takada; Yukisumi Iizuka; Kunio Itoh; Yorihisa Tanaka “Effects of zinc deficiency on rat liver drug-metabolizing enzymes”
    Journal of Tohoku Phamaceutical University. 2005, 52, 85-97.

21 Pharmaceutical Sciences

Research Projects

  • Yoshikawa,S., Murata,R., Shida,S., Uwai,K., Suzuki,T., Katsumata,S., Takeshita,M.
    “Evaluation of correlation between dissolution rates of loxoprofen tablets and their surface morphology observed by scanning electron microscope and atomic force microscope”
    Chem. Pharm.Bull. 2010, 58, 34-37.
  • Sato,K., Suzuki,T., Nakamura,H., Ishikawa,M., Tanaka,Y., Hayashi,T.
    “Utilization of the network tool for progression of practical training for pharmacy in Tohoku Pharmaceutical University”
    J. Drug Interact. Res. 2010, 34, 47-51.
  • Yoshikawa,S., Suzuki,U., Murata,R., Yoshida,S., Uwai,K., Suzuki,T., Takeshita,M.
    “Clinical effect of once an intake of generic loxoprofen tablets”
    Pain Clinic. 2010, 31, 635-640.
  • Masuda,S., Oka,R., Uwai,K., Matsuda,Y., Shiraishi,T., Nakagawa,Y., Shoji,T., Mihara,C., Takeshita,M., Ozawa,K.
    “Development of clinical application for a nutritional prescription support system for total parenteral/enteral nutrition”
    Yakugaku Zasshi. 2009, 129 1077-1086
  • Uwai,K.,Osanai,Y., Imaizumi,T., Kanno,S., Takeshita,M., Ishikawa,M.
    “Inhibitory effect of the alkyl side chain of caffeic acid analogues on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages”
    Bioorg. Med. Chem. 2008, 16, 7795-7803.
  • Osanai,Y., Kanno,S., Uwai,K., Takeshita,M., Ishikawa,M.
    “Inhibitory effect of octylcaffeate on lipopolysaccharide-induced nitric oxide production in macrophages”
    Pharmacometrics. 2008, 75, 19-24.
  • Uwai,K., Konno,N., Yasuta,Y., Takeshita,M.
    “Electronic effect of para-subustitution on acetophenones in the reaction of rat liver 3 -hydroxysteroid dehydrogenase”
    Bioorg. Med. Chem. 2008, 16, 1084-1089.
  • Oka,R., Uwai,K., Toyoguchi,T., Shoji,T., Shiraishi,T., Nakagawa,Y., Takeshita,M.
    “Stability of magnesium oxide product stored in single-dose packages”
    Jpn. J. Pharma. Health Care Sci. 2007, 33, 1013-1019.
  • Uwai,K., Konno,N., Kitamura,S., Ohta,S., Takeshita,M.
    “Purification and characterization of rat liver enzyme catalyzing stereoselective reduction of acetylpyridines”
    Chirality. 2005, 17, 494-500.
  • Uwai,K., Tani,M., Ohtake,Y., Abe,S., Maruko,A., Chiba,T., Hamaya,Y., Ohkubo,Y., Takeshita,M.
    “Photodegradation products of propranolol: the structures and pharmacological studies”
    Life Sci. 2005, 78, 357-65.
  • Uwai,K., Uchiyama,H., Sakurada,S., Kabuto,C., Takeshita,M.
    “Syntheses and receptor-binding studies of derivatives of the opioid antagonist naltrexone”
    Bioorg. Med. Chem. 2004, 12, 417-21.

22 Clinical Pharmacotherapeutics

Research Projects

  • Kanno, S.; Itoh, K.; Suzuki, N.; Tomizawa, A.; Yomogida, S.; Masaaki Ishikawa, M. “Exogenous albumin inhibits sorafenib-induced cytotoxicity in human cancer cell lines.”
    Mol. Cli. Oncol., 2013, 200, 1, 29-34.
  • Tomizawa, A.; Kanno, S.; Osanai, Y.; Goto, A.; Sato, C.; Yomogida, S.; Ishikawa, M. “Induction of apoptosis by a potent caffeic acid derivative, caffeic acid undecyl ester, is mediated by mitochondrial damage in NALM-6 human B cell leukemia cells.”
    Oncol. Rep., 2013, 200, 29(2), 425-9.
  • Tomizawa, A.; Kanno, S.; Osanai, Y.; Yomogida, S.; Ishikawa, M. “Cytotoxic effects of caffeic acid undecyl ester are involved in the inhibition of telomerase activity in NALM-6 human B-cell leukemia cells”
    Oncol. Lett., 2013, 6(4), 875-877.
  • Kanno, S.; Yomogida, S.; Tomizawa, A.; Yamazaki, H.; Ukai, K.; Mangindaan, R. E. P.; Namikoshi, M.; Ishikawa, M. “Papuamine causes autophagy following the reduction of cell survival through mitochondrial damage and JNK activation in MCF-7 human breast cancer cells”
    Int. J. Oncol., 2013, 43(5), 1413-9.
  • Watanabe, K.; Kanno, S.; Tomizawa, A.; Yomogida, S.; Ishikawa, M. “Acacetin induces apoptosis in human T cell leukemia Jurkat cells via activation of a caspase cascade”
    Oncol. Rep., 2012, 27(1), 204-9.
  • Kanno, S.; Maeda, N.; Tomizawa, A.; Yomogida, S.; Katoh, T.; Ishikawa, M. “Involvement of p21waf1/cip1 expression in the cytotoxicity of the potent histone deacetylase inhibitor spiruchostatin B towards susceptible NALM-6 human B cell leukemia cells”
    Int. J. Oncol., 2012, 40(5), 1391-6.
  • Kanno, S.; Maeda, N.; Tomizawa, A.; Yomogida, S.; Katoh, T.; Ishikawa, M. “Characterization of cells resistant to the potent histone deacetylase inhibitor spiruchostatin B (SP-B) and effect of overexpressed p21waf1/cip1 on the SP-B resistance or susceptibility of human leukemia cells”
    Int. J. Oncol., 2012, 41(3), 862-8.
  • Kanno, S.; Kurauchi, K.; Tomizawa, A.; Yomogida, S.; Ishikawa, M. “Albumin modulates docosahexaenoic acid-induced cytotoxicity in human hepatocellular carcinoma cell lines.”
    Toxicol. Lett. 2011, 200, 154-161.
  • Kanno, S.; Kitajima, Y.; Kakuta, M.; Osanai, Y.; Kurauchi, K.; Ujibe, M.; Ishikawa, M. “Costunolide-induced apoptosis is caused by receptor-mediated pathway and inhibition of telomerase activity in NALM-6 cells.”
    Biol. Pharm. Bull. 2008, 31, 1024-1028.
  • Kanno, S.; Ohtake, T.; Osanai, Y.; Kurauchi, K.; Ujibe, M.; Uwai, K.; Takeshita, M.; Satoh, S.; Ishikawa, M. “Trimidox-induced apoptosis is mediated through induction of p53 in NALM-6 cells.”
    J. Pharmacol. Sci. 2008, 106, 521-524.
  • Kanno, S.; Hiura, T.; Shouji, A.; Osanai, Y.; Ujibe, M.; Ishikawa, M. “Resistance to Ara-C up-regulates the activation of NF-kappaB, telomerase activity and Fas expression in NALM-6 cells.”
    Biol. Pharm. Bull. 2007, 30, 2069-2074.
  • Kanno, S.; Kakuta, M.; Kitajima, Y.; Osanai, Y.; Kurauchi, K.; Ohtake, T.; Ujibe, M.; Uwai, K.; Takeshita, M.; Ishikawa, M. “Inhibitory effect of trimidox on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages.”
    J. Pharmacol. Sci. 2007, 104, 278-281.
  • Kanno, S.; Kakuta, M.; Kitajima, Y.; Osanai, Y.; Kurauchi, K.; Ohtake, T.; Ujibe, M.; Uwai, K.; Takeshita, M.; Ishikawa, M. “Preventive effect of trimidox on oxidative stress in U937 cell line.”
    Biol. Pharm. Bull. 2007, 30, 994-998.
  • Kanno, S.; Hiura, T.; Ohtake, T.; Koiwai, K.; Suzuki, H.; Ujibe, M.; Ishikawa M. “Characterization of resistance to cytosine arabinoside (Ara-C) in NALM-6 human B leukemia cells.”
    Clin. Chim. Acta. 2007, 377, 144-149.
  • Kanno, S.; Tomizawa, A.; Ohtake, T.; Koiwai, K.; Ujibe, M.; Ishikawa, M. “Naringenin-induced apoptosis via activation of NF-kappaB and necrosis involving the loss of ATP in human promyeloleukemia HL-60 cells.”
    Toxicol. Lett. 2006, 166, 131-139.
  • Kanno, S.; Tomizawa, A.; Hiura, T.; Osanai, Y.; Kakuta, M.; Kitajima, Y.; Koiwai, K.; Ohtake, T.; Ujibe, M.; Ishikawa, M. “Melatonin protects on toxicity by acetaminophen but not on pharmacological effects in mice.”
    Biol. Pharm. Bull. 2006, 29, 472-476.
  • Kanno, S.; Uwai, K.; Tomizawa, A.; Hiura, T.; Osanai, Y.; Ujibe, M.; Takeshita, M.; Ohtake, T.; Kimura, K.; Ishikawa, M. “Trimidox induces apoptosis via cytochrome c release in NALM-6 human B cell leukaemia cells.”
    Basic Clin. Pharmacol. Toxicol. 2006, 98, 44-50.
  • Ujibe, M.; Kanno, S.; Osanai, Y.; Koiwai, K.; Ohtake, T.; Kimura, K.; Uwai, K.; Takeshita, M.; Ishikawa, M. “Octylcaffeate induced apoptosis in human leukemia U937 cells.”
    Biol. Pharm. Bull. 2005, 28, 2338-2341.
  • Kanno, S.; Shouji, A.; Tomizawa, A.; Hiura, T.; Osanai, Y.; Ujibe, M.; Obara, Y.; Nakahata, N.; Ishikawa, M. “Inhibitory effect of naringin on lipopolysaccharide (LPS)-induced endotoxin shock in mice and nitric oxide production in RAW 264.7 macrophages.”
    Life Sci. 2006, 78, 673-681.
  • Kanno, S.; Tomizawa, A.; Hiura, T.; Osanai, Y.; Shouji, A.; Ujibe, M.; Ohtake, T.; Kimura, K.; Ishikawa, M. “Inhibitory effects of naringenin on tumor growth in human cancer cell lines and sarcoma S-180-implanted mice.”
    Biol. Pharm. Bull. 2005, 28, 527-530.
  • Kanno, S.; Higurashi, A.; Watanabe, Y.; Shouji, A.; Asou, K.; Ishikawa, M. “Susceptibility to cytosine arabinoside (Ara-C)-induced cytotoxicity in human leukemia cell lines.”
    Toxicol. Lett. 2004, 152, 149-158.
  • Kanno, S.; Shouji, A.; Hirata, R.; Asou, K.; Ishikawa, M. “Effects of naringin on cytosine arabinoside (Ara-C)-induced cytotoxicity and apoptosis in P388 cells.” Life Sci. 2004, 75, 353-365.
  • Aso, K.; Kanno, S.; Tadano, T.; Satoh, S.; Ishikawa, M. “Inhibitory effect of propolis on the growth of human leukemia U937.”
    Biol. Pharm. Bull. 2004, 27, 727-730.
  • Kanno, S.; Matsukawa, E.; Miura, A.; Shouji, A.; Asou, K.; Ishikawa, M. “Diethyldithiocarbamate-induced cytotoxicity and apoptosis in leukemia cell lines.”
    Biol. Pharm. Bull. 2003, 26, 964-968.
  • Kanno, S.; Shouji, A.; Asou, K.; Ishikawa, M. “Effects of naringin on hydrogen peroxide-induced cytotoxicity and apoptosis in P388 cells.”
    J. Pharmacol. Sci. 2003, 92, 166-170.

23 Division of Regulatory Glycobiology

Research Projects

  • Gu, W.; Fukuda, T.; Isaji, T.; Hashimoto, H.; Wang., Y. and Gu, J. α1,6-Fucosylation regulates neurite formation via the activin/phospho-Smad2 pathway in PC12 cells: the implicated dual effects of Fut8 for TGF-β/activin-mediated signaling.
    FASEB J., 2013, 27:3947-58.
  • Xu, Q.; Isaji, T.; Lu Y.; Gu, W.; Kondo, M.; Fukuda, T.; Du, Y. and Gu, J. Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by TGF-β1 in epithelial cell lines.
    J. Biol. Chem., 2012, 287:16563-74.
  • Li, W.; Liu, Q.; Pang, Y.; Jin, J.; Wang, H.; Cao, H.; Li, Z.; Wang, X.; Ma, B.; Chi, Y.; Wang, R.; Kondo, A.; Gu, J. and Taniguchi, N. Core fucosylation of mu heavy chains regulates the assembly of precursor B cell receptors and its intracellular signaling.
    J. Biol. Chem., 2012, 287:2500-8.
  • Fukuda, T.; Hashimoto, H.; Okayasu, N.; Kameyama, A.; Onogi, H.; Nakagawasai, O, Nakazawa, T.; Kurosawa, T;, Hao, Y.; Isaji, T.; Tadano, T.; Narimatsu, H.; Taniguchi, N. and Gu, J. “α1,6-Fucosyltransferase-deficient mice exhibit multiple behavioral abnormalities associated with a schizophrenia-like phenotype: importance of the balance between the dopamine and serotonin systems”
    J. Biol. Chem. 2011, 286, 18434-43.
  • Xu, Q.; Akama, R.; Isaji, T.; Lu, Y.; Hashimoto, H.; Kariya, Y.; Fukuda, T.; Du Y.; and Gu, J. “Wnt/β-catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression:The implication of two mutually exclusive pathways for regulation”
    J. Biol. Chem. 2011, 286, 4310-8.
  • Kariya, Y.; Kawamura, C.; Tabei, T.; Gu, J. “Bisecting GlcNAc residues on laminin-332 downregulates galectin-3 dependent keratinocyte motility”
    J. Biol. Chem. 2010, 285, 3330-3340.
  • Sato, Y.; Isaji, T.; Tajiri, M.; Yoshida-Yamamoto, S.; Yoshinaka, T.; Somehara, T.; Fukuda, T.; Wada, Y. and Gu, J. “An N-glycosylation site on the β-propeller domain of the integrin α5 subunit plays key roles in both its function and site-specific modification by β1,4-N-acetylglucosaminyltransferase III”
    J. Biol. Chem. 2009, 284, 11873-81.
  • Isaji, T.; Sato, Y.; Fukuda, T.; and Gu, J. “N-glycosylation of the I-like domain of beta1 integrin is essential for beta1 integrin expression and biological function: Identification of the minimal N-glycosylation requirement for alpha 5beta 1”
    J. Biol. Chem. 2009,284,12207-16.
  • Kariya, Y.; Kato, R.; Itoh, S.; Fukuda, T.; Shibukawa, Y.; Sanzen, N.; Sekiguchi, K.; Wada, Y.; Kawasaki, N.; Gu, J. “N-glycosylation of laminin-332 regulates its biological functions: A novel function of the bisecting GlcNAc”
    J. Biol. Chem. 2008, 283, 33036-33045.
  • Kotani, N.; Gu, J.; Isaji, T.; Udaka, K.; Taniguchi, N.; and Honke, K. “Biochemical visualization of cell surface molecular clustering in living cells”
    Proc. Natl. Acad. Sci. 2008, 105, 7405-9.
  • Matsumura, K.; Higashida, K.; Ishida, H.; Hata, Y.; Abe, Y.; Kato, M.; Ueda, M.; Yamamoto, K.; Shigeta, M.; Mizuno, Y.; Miyoshi, E.; Gu, J. and Taniguchi, N. “Carbohydrate-binding specificity of a fucose-specific lectin from Aspergillus Oryzae: a novel probe for core fucose”
    J. Biol. Chem. 2007, 282, 15700-8.
  • Zhao, Y.; Itoh, S.; Wang, X.; Isaji, T.; Miyoshi, E.; Kariya, Y.; Kondo, A.; Miyazaki, K.; Kawasaki, N.; Taniguchi, N.; and Gu, J. “Deletion of core fucosylation on α3β1 integrin down-regulates its functions”
    J. Biol. Chem. 2006, 281, 38343-38350.
  • Zhao, Y.; Nakagawa, T.; Itoh, S.; Inamori, K. I.; Isaji, T.; Kariya, Y.; Kondo, A.; Miyoshi, E.; Miyazaki, K.; Kawasaki, N.; Taniguchi, N.; and Gu, J. “N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on α3β1 integrin-mediated cell migration”
    J. Biol. Chem. 2006, 281, 32122-32130.
  • Isaji, T.; Sato, Y.; Zhao, Y.; Miyoshi, E.; Wada, Y.; Taniguchi, N. and Gu, J. “N-glycans on the β-propeller domain of the integrin α5 subunit are essential for α35β1 heterodimerization, expression on the cell surface and its biological function”
    J. Biol. Chem. 2006, 281, 33258-33267.
  • Iijima, J.; Zhao, Y.; Isaji, T.; Kameyama, A.; Nakaya, S.; Wang, X.; Ihara, H.; Cheng, X.; Nakagawa, T.; Miyoshi, E.; Kondo, A.; Narimatsu, H.; Taniguchi, N. and Gu, J. “Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells: involvement of E-cadherin-mediated cell adhesion”
    J. Biol. Chem. 2006, 281, 13038-46.
  • Wang X; Gu J; Ihara H; Miyoshi E; Honke K; Taniguchi N. “Core fucosylation regulates EGF receptor-mediated intracellular signaling”
    J. Biol. Chem. 2006, 281, 2572-7.
  • Wang, X.; Inoue ,S.; Gu ,J.; Miyoshi, E.; Noda, K.; Li, W.; Mizuno-Horikawa ,Y.; Nakano ,M.; Asahi, M.; Takahashi, M.; Uozumi, N.; Ihara, S.; Lee, SH.; Ikeda, Y.; Yamaguchi, Y.; Aze, Y.; Tomiyama, Y.; Fujii, J.; Suzuki, K.; Kondo, A.; Shapiro, SD.; Lopez-Otin, C.; Kuwaki T.; Okabe, M.; Honke, K.; Taniguchi, N. “Dysregulation of TGF-β1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice”
    Proc. Natl. Acad. Sci. 2005, 102, 15791-6.
  • Isaji, T.; Gu, J.; Nishiuchi, R.; Zhao, Y.; Takahashi, M.; Miyoshi, E.; Honke, K.; Sekiguchi, K.; and Taniguchi, N. “Introduction of bisecting GlcNAc into integrin α5β1 reduces ligand binding and down-regulates cell adhesion and cell migration”
    J. Biol. Chem. 2004, 279, 19747-54.
  • Inamori, KI.; Endo, T.; Gu, J.; Matsuo, I.; Ito, Y.; Fujii, S.; Iwasaki, H.; Narimatsu, H.; Miyoshi, E.; Honke, K.; and Taniguchi, N. “N-acetylglucosaminyltransferase IX acts on the GlcNAcβ1,2-Manalpha 1-Ser/Thr moiety, forming α2,6-branched structure in brain O-mannosyl glycan”
    J. Biol. Chem. 2004, 279, 2337-2340.
  • Noda, K.; Miyoshi, E.; Gu, J.; Gao, C-X.; Nakahara, S.; Kitada, T.; Honke, K.; Suzuki, K.; Yoshihara, H.; Yoshikawa, K.; Kawano, K.; Tonetti, M.; Kasahara, A.; Hori, M.; Hayashi, N.; and Taniguchi, N. “Relationship between elevated FX expression and increased production of GDP-L-fucose, a common donor substrate for fucosylation in human hepatocellular carcinoma and hepatoma cell lines”
    Cancer Res. 2003, 63, 6282-9.
  • Shigeta, M.; Sanzen, N.; Ozawa, M.; Gu, J.; Hasegawa, H.; and Sekiguchi, K. “CD151 regulates epithelial cell-cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization”
    J. Cell Biol. 2003, 163, 165-176.

24 Division of Cell Recognition Study

Research Projects

  • Tatsuta T.; Hosono M.; Takahashi K.; Omoto T.; Kariya Y.; SugawaraS.; Hakomori S.; Nitta K. “Sialic acid-binding lectin (leczyme) induces apoptosis to malignant mesothelioma and exerts synergistic anti-tumor effect with TRAIL”
    Int. J. Oncol., 2014, 44, 377-384.
  • Tatsuta T.; Hosono M.; Ogawa Y.; Inage K.; Sugawara S.; Nitta K. “Down-regulation of Hsp70 inhibits apoptosis induced by sialic acid-binding lectin (leczyme)”
    Oncol. Rep., 2014, 31, 13-18.
  • Tatsuta T.; Hosono M.; Miura Y.; Sugawara S.; Hakomori S.; Nitta K. “Involvement of ER stress in apoptosis induced by sialic acid-binding lectin (leczyme) from bullfrog eggs”
    Int. J. Oncol., 2013, 43, 1799-1808.
  • Tatsuta T.; Hosono M.; Sugawara S.; Kariya Y.; Ogawa Y.; Hakomori S.; Nitta K. “Sialic acid-binding lectin (leczyme) induces caspase-dependent apoptosis- mediated mitochondrial perturbation in Jurkat cells”
    Int. J. Oncol., 2013, 43, 1402-1412.
  • Hosono M.; Sugawara S.; Tatsuta T.; Hikita T.; Kominami J.; Sachiko N.T.; Hirabayashi J.; Kawsar S.M.A.; Ozeki Y.; Hakomori S.I.; Nitta K. “Domain Composition of Rhamnose-binding Lectin from Shishamo smelt eggs and its Carbohydrate-binding Profiles”
    Fish Physiol. Biochem., 2013, 39, 1619-1630.
  • Ambo A.; Ohkatsu H.; Minamizawa M.; Watanabe H.; Sugawara S.; Nitta K.; Tsuda Y., Okada Y.; Sasaki Y. “Relationship between structure and P-glycoprotein inhibitory activity of dimeric peptides related to the Dmt-Tic pharmacophore”
    Bioorg Med Chem Lett., 2012, 22, 2192-2194.
  • Koseki K.; Wada T.; Hosono M.; Hata K.; Yamaguchi K.; Nitta K.; Miyagi T. “Human cytosolic sialidase NEU2-low general tissue expression but involvement in PC-3 prostate cancer cell survival”
    Biochem. Biophys. Res. Commum., 2012, 428, 142-149.
  • Takahashi K.; Mitoma J.; Hosono M., Shiozaki K.; Sato C.; Yamaguchi K.; Kitajima K.; Higashi H.; Nitta K.; Shima H.; Miyagi T. “Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons”
    J. Biol. Chem., 2012, 287, 14816-14826.
  • Fujii Y.; Dohmae N.; Takio K.; Kawsar S.M.A.; Matsumoto R.; Hasan I.; Koide Y.; Kanaly R.A.; Yasumitsu H.; Ogawa Y.; Sugawara S.; Hosono M.; Nitta K.; Hamako J.; Matsui T.; Ozeki Y. “A lectin from the mussel Mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells”
    J. Biol. Chem., 2012, 287, 44772-44783.
  • Matsumoto R.; Fujii Y.; Kawsar S.M.A.; Kanaly R.A.; Yasumitsu H.; Koide Y.; Hasan I.; Iwahara C.; Ogawa Y.; Im C.H.; Sugawara S.; Hosono M.; Nitta K.; Hamako J.; Matsui T.; Ozeki Y. “Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai”
    Toxins, 2012, 4, 323-338.
  • Fujii Y.; Sugawara S.; Araki D.; Kawano T.; Tatsuta T.; Takahashi K.; Kawsar S.M.; Matsumoto R.; Kanaly R.A.; Yasumitsu H.; Ozeki Y.; Hosono M.; Miyagi T.; Hakomori S.I.; Takayanagi M.; Nitta K. “MRP1 expressed on Burkitt’s lymphoma cells was depleted by catfish egg lectin through Gb3-glycosphingolipid and enhanced cytotoxic effect of drugs”
    Protein J., 2012, 31, 15-26.
  • Kawsar S.M.A.; Matsumoto R.; Fujii Y.; Matsuoka H.; Matsuda N.; Iwahara C.; Yasumitsu H.; Kanaly R.A.; Sugawara S.; Hosono M.; Nitta K.; Ishizaki N.; Dogasaki C.; Hamako J.; Matsui T.; Ozeki Y. “Cytotoxicity and glycan-binding profile of a D-galactose-binding lectin from the eggs of a Japanese sea hare (Aplysia kurodai)”
    Protein J., 2011, 30, 509-519.
  • Fujii Y.; Kawsar S.M.; Matsumoto R.; Yasumitsu H.; Ishizaki N.; Dogasaki C.; Hosono M.; Nitta K.; Hamako J.; Matsui T.; Ozeki Y. “A D-galactose-binding lectin purified from coronate moon turban, Turbo (Lunella) coreensis, with a unique amino acid sequence and the ability to recognize lacto-series glycosphingolipids”
    Comp. Biochem. Physiol. B, 2011,158, 30-37.
  • Kawsar S.M.; Matsumoto R.; Fujii Y.; Yasumitsu H.; Dogosaki C.; Hosono M.; Nitta K.; Hamako J.; Matsui T.; Ozeki Y. “Purification and biochemical characterization of a D-galactose binding lectin from Japanese sea hare (Aplysia kurodai) eggs”
    Biochemistry (Moscow), 2009, 74, 709-716
  • Kawsar S.M.; Matsumoto R.; Fujii Y.; Yasumitsu H.; Uchiyama H.; Hosono M.; Nitta K.; Hamako J.; Matsui T.; Kojima N.; Ozeki Y. “Glycan-binding profile and cell adhesion activity of American bullfrog (Rana catesbeiana) oocyte galectin-1”
    Protein Pept. Lett., 2009, 16, 677-684
  • Kawano T,; Sugawara S.; Hosono M.; Tatsuta T.; Ogawa Y.; Fujimura T.; Taka H.; Murayama K.; Nitta K. “Globotriaosylceramide-expressing Burkitt’s lymphoma cells are committed to early apoptotic status by rhamnose-binding lectin from catfish eggs”
    Biol. Pharm. Bull., 2009, 32, 345-353.
  • Sugawara S.; Kawano T.; Omoto T.; Hosono M.; Tatsuta T.; Nitta K. “Binding of Silurus asotus lectin to Gb3 on Raji cells causes disappearance of membrane-bound form of HSP70”
    Biochim. Biophys. Acta, 2009, 1790, 101-109.
  • Kawsar S.M.; Fujii Y.; Matsumoto R.; Ichikawa T.; Tateno H.; Hirabayashi J.; Yasumitsu H.; Dogasaki C.; Hosono M.; Nitta K.; Hamako J.; Matsui T.; Ozeki Y. “Isolation, purification, characterization and glycan-binding profile of a d-galactoside specific lectin from the marine sponge, Halichondria okadai”
    Comp. Biochem. Physiol. B, 2008, 150, 349-357.
  • Sasaki Y.; Minamizawa M.; Ambo A.; Sugawara S.; Ogawa Y.; Nitta K. “Cell-penetrating peptide-conjugated XIAP-inhibitory cyclic hexapeptides enter into Jurkat cells and inhibit cell proliferation”
    FEBS J., 2008, 275, 6011-6021.
  • Kawano T.; Sugawara S.; Hosono M.; Tatsuta T.; Nitta K. “Alteration of gene expression induced by Silurus asotus lectin in Burkitt’s lymphoma cells.”
    Biol. Pharm. Bull., 2008, 31, 998-1002.
  • Nitta K.; Kawano T.; Sugawara S.; Hosono M. “Regulation of globotriaosylceramide (Gb3)-mediated signal transduction by rhamnose-binding lectin”
    Yakugaku Zasshi, 2007, 127, 553-561.
  • Hosono M.; Sugawara S.; Ogawa Y.; Kohno T.; Takayanagi M.; Nitta K. “Purification, characterization, cDNA cloning, and expression of asialofetuin-binding C-type lectin from eggs of shishamo smelt (Osmerus [Spirinchus] lanceolatus)”
    Biochim. Biophys. Acta, 2005, 1725, 160-173.
  • Sugawara S.; Hosono M.; Ogawa Y.; Takayanagi M.; Nitta K. “Molecular and sugar-binding heterogeneity of C-type lectins from Osmerus (Spirinchus) lanceolatus eggs”
    Biol. Pharm. Bull., 2005, 28, 791-796.
  • Sugawara S.; Hosono M.; Ogawa Y.; Takayanagi M.; Nitta K. “Catfish egg lectin causes rapid activation of multidrug resistance 1 P-glycoprotein a lipid translocase”
    Biol. Pharm. Bull., 2005, 28, 434-441.
  • Sugawara S.; Sasaki S.; Ogawa Y.; Hosono M.; Nitta K. “Catfish (Silurus asotus) lectin enhances the cytotoxic effects of doxorubicin”
    Yakugaku Zasshi, 2005, 125, 327-334.
  • Hosono M.; Sugawara S.; Ogawa Y.; Takayanagi M.; Nitta K. “DNA aggregating protein from carp (Cyprinus capio) eggs”
    Biol. Pharm. Bull., 2005, 28, 13-18.
  • Carbohydrate structure and differential binding of prostate specific antigen to Maackia amurensis lectin between prostate cancer and benign prostate hypertrophy.
    Ohyama C,; Hosono M.; Nitta K.; Oh-eda M.; Yoshikawa K.; Habuchi T.; Arai Y.; Fukuda M.
    Glycobiology, 2004, 14, 671-679.

25 Division of Glycopathology

Research Projects

  • Uemura S, Go S, Shishido F, Inokuchi J (2013) Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells. Glycoconj. J. DOI 10.1007/s10719-013-9499-1
  • Inokuchi J, Nagafuku M, Ohno I. and Suzuki A. Heterogeneity of gangliosides among T cell subsets. (2013) Cell. Mol. Life Sci. 70, 3067-75
  • Nagafuku M, Inokuchi J, The Physiological Significance of Ganglioside Species Selectively Expressed on Individual T Cell Subsets. Trends in Glycoscience and Glycotechnology 25, 159-169 (2013)
  • Nagafuku M, Inokuchi J, The Physiological Significance of Ganglioside Species Selectively Expressed on Individual T Cell Subsets. Trends in Glycoscience and Glycotechnology 25, 159-169 (2013)
  • Nagafuku M, Okuyama K., Onimaru Y., Suzuki A., Odagiri Y., Yamashita T., Iwasaki K., Fujiwara M., Takayanagi M., Ohno I. and Inokuchi J.
    CD4 and CD8 T cells require different membrane gangliosides for activation.
    (2012) Proc. Natl. Acad. Sci.USA 109. E336-E342
    Author summary 109. 1832-1833.
  • Sekimoto J., Kabayama K., Gohara K., and Inokuchi J.
    Dissociation of the insulin receptor from caveolae during TNFa-induced insulin resistance and its recovery by D-PDMP. (2012)
    FEBS Lett. 586, 191-195
  • Inokuchi, J. “Physiopathological function of hematoside (GM3 ganglioside)”
    Proc. Jpn. Acad., Ser. B 2011, 87, 179-198
  • Go, S.; Yoshikawa, M.; Inokuchi, J. “Glycoconjugates in the mammalian and auditory system”
    J. Neurochem. 2011, 5, 756-763
  • Inokuchi, J. “Membrane Microdomains and Insulin Resistance “
    FEBS letters 2010, 584, 1864-1871
  • Uemura, S.; Yoshida, S.; Shishido, F.; Inokuchi, J. “The Cytoplasmic tail of GM3 synthase defines its subcelluar localization, stability, and in vivo activity.”
    Mol. Biol. Cell 2009, 20, 3088-3100
  • Yoshikawa, M.; Go, S.; Takasaki, K.; Kakazu, Y.; Ohashi, M.; Nagafuku, M.; Kabayama, K.; Sekimoto, J.; Suzuki, S.; Takaiwa, K.; Kimitsuki, T.; Matsumoto, N.; Komune, S.; Kamei, D.; Saito, M.; Fujiwara, M.; Iwasaki, K.; Inokuchi, J. “Mice lacking ganglioside GM3 synthase exhibit complete hearing loss due to selective degeneration of the organ of Corti”
    Proc. Natl. Acad. Sci.USA 2009, 106, 9483-9488
  • Sato, T.; Nagafuku, M.; Shimizu, K.; Taira, T.; Igarashi, Y.; Inokuchi, J. “Physiological levels of insulin and IGF-1 synergistically enhance the differentiation of mesenteric adipocytes”
    Cell Biol. Int. 2008, 32, 1397-1404
  • Sato, T.; Nihei, Y.; Nagafuku, M.; Tagami, S.; Chin, R.; Kawamura, M.; Miyazaki, S.; Suzuki, M.; Sugahara, S.; Takahashi, Y.; Saito, A.; Igarashi, Y; Inokuchi, J. “Circulating Levels of Ganglioside GM3 in Metabolic Syndrome: A Pilot Study”
    Obesity Research & Clinical Practice. 2008, 2, 231-238
  • Kabayama, K.; Sato, T.; Saito, K.; Loberto, N.; Prinetti, A.; Sonnino, S.; Kinjo, M.; Igarashi, Y.; Inokuchi, J. “Dissociation of the insulin receptor and caveolin-1 complex by ganglioside GM3 in the state of insulin resistance”
    Proc. Natl. Acad. Sci.USA, 2007, 104, 13678-13683
  • Noguchi, M.; Suzuki, T.; Kabayama, K.; Takahashi, H.; Chiba, H.; Shiratori, M.; Abe, S.; Watanabe, A.; Satoh, M.; Hasegawa, T.; Tagami, S.; Ishii, A.; Saito, M.; Kaneko, M.; Iseki, K.; Igarashi, Y.; Inokuchi, J. “GM3 synthase gene is a novel biomarker for histological classification and drug sensitivity against epidermal growth factor receptor tyrosine kinase inhibitors in non-small cell lung cancer”
    Cancer Science 2007, 98, 1625-1632
  • Uemura, S.; Fei, Feng.; Kume, M.; Yamada, K.; Kabayama, K.; Nishimura, S.; Igarashi, Y; Inokuchi, J. “Cell Growth Arrest by Sialic Acid Clusters in Ganglioside GM3 Mimetic Polymers”
    Glycobiology 2007, 17,568-577
  • Uemura, S.; Kihara, A.; Iwaki, S.; Inokuchi, J.; Igarashi, Y. “Regulation of the transport and protein levels of the inositol phosphorylceramide mannosyltransferases Csg1 and Csh1 by the Ca2+- binding protein Csg2”
    J. Biol. Chem. 2007, 82, 8613-8621
  • Noguchi, M.; Kabayama, K.; Uemura, S.; Kang, BW.; Saito, M.; Igarashi, Y.; Inokuchi, J. “Endogenously Produced Ganglioside GM3 Endows Etoposide and Doxorubicin Resistance by Up-regulating Bcl-2 Expression in 3LL Lewis Lung Carcinoma Cells”
    Glycobiology 2006, 16, 641-650
  • Uemura, S.; Kurose, T.; Suzuki, T.; Yoshida, S.; Ito, M.; Saito, M.; Igarashi, Y.; Inokuchi, J. “Substitution of the N-glycan Function in Glycosyltransferases by Specific Amino Acids (SUNGA): ST3Gal-V as a model enzyme”
    Glycobiology 2006, 16, 258-270
  • Sato, T.; Zakaria, A.M.; Uemura, S.; Ishii, A.; Ohno-Iwashita, Y.; Igarashi, Y; Inokuchi, J. “Role for up-regulated ganglioside biosynthesis and association of Src family kinases with microdomains in retinoic acid-induced differentiation of F9 embryonal carcinoma cells”
    Glycobiology 2005, 15, 687-699
  • Kabayama, K.; Sato, T.; Kitamura, F.; Uemura, S.; Kang, B.W.; Igarashi, Y.; Inokuchi, J. “TNF alpha-induced insulin resistance in adipocytes as a membrane microdomain disorder:involvement of ganglioside GM3”
    Glycobiology 2005, 15, 21-29
  • Nagafuku, M.; Kabayama, K.; Oka, D.; Kato, A.; Tani-ichi, S.; Shimada, Y.;
    Ohno-Iwashita, Y.; Yamasaki, S.; Saito, T.; Iwabuchi, K.; Hamaoka, T.; Inokuchi, J.; Kosugi, A. “Reduction of Glycosphingolipid Levels in Lipid Rafts Affects the Expression State and Function of Glycosylphosphatidyl-inositol-anchored Proteins but Does Not Impair Signal Transduction via the T Cell Receptor”
    J. Biol. Chem. 2003, 278, 51920-51927
  • Yamagishi, K.; Ohgami, Y.; Iwasaki, K.; Jimbo, M.; Masuda, H.; Fujiwara, M.; Igarashi, Y.; Inokuchi, J. “A synthetic ceramide analog ameliorated memory defficits and stimulates brain ganglioside synthesis in ischemic rats”
    Eur. J. Pharmacol. 2003, 462, 53-60
  • Uemura, S.; Kabayama, K.; Noguchi, M.; Igarashi, Y.; Inokuchi, J. “Sialylation and Sulfation od Lactosylceramide Distinctly Regulate Anchorage-Independent Growth, Apoptosis and Gene Expression in 3LL Lewis Lung Carcinoma Cells
    Glycobiology 2003, 13, 207-216
  • Igarashi, K.; Furuse, H.; Fujii, S.; Ito, K.; Kaneko, K.; Kato, H.; Waki, H,; Ando, S.;
    Inokuchi, J. “Effects of mono- and tetra-sialogangliosides, GM1 and GQ1b on ATP-induced long-term potentiation in hippocampal CA1 neurons”
    Glycobiology 2002, 12, 339-344
  • Tagami, S.; Inokuchi, J.; Kabayama, K.; Yoshimura, H.; Kitamura, F.; Uemura, S.; Ogawa, C.; Ishii, A.; Saito, M.; Ohtsuka, Y.; Sakaue, S.; Igarashi, Y. “Ganglioside GM3 Participates in the Pathological Conditions of Insulin Resistance”
    J. Biol. Chem. 2002, 277, 3085-3092

26 Division of Glyco-Signal Research

Research Projects

  • Watanabe, S., Tan-No, K., Tadano, T., Higashi, H. “Intraplantar injection of gangliosides produces nociceptive behavior and hyperalgesia via a glutamate signaling mechanism”
    Pain 2011, 152,327-34.
  • Takara, T., Nakagawa, T., Isobe, M., Okino, N., Ichinose, S., Omori, A., Ito, M. “Purification, molecular cloning, and application of a novel sphingomyelin-binding protein (clamlysin) from the brackishwater clam, Corbicula japonica”
    Biochim Biophys Acta 2011, 1811,323-32.
  • Nakagawa, T., Tani, M., Sueyoshi, N., Ito, M. “The mucin box and signal/anchor sequence of rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membrane”
    Bioscience, Biotechnology, and Biochemistry 2011, 75,987-90.
  • Kobayashi, M., Mitoma, J., Hoshino, H., Yu, S.Y., Shimojo, Y., Suzawa, K., Khoo, K.H., Fukuda, M., Nakayama, J. “Prominent expression of sialyl Lewis X-capped core 2-branched O-glycans on high endothelial venule-like vessels in gastric MALT lymphoma”
    Journal of Pathology 2011, 224,67-77.
  • Watanabe, S., Endo, S., Oshima, E., Hoshi, T., Higashi, H., Yamada, K., Tohyama, K., Yamashita, T., Hirabayashi, Y. “Glycosphingolipid synthesis in cerebellar Purkinje neurons: roles in myelin formation and axonal homeostasis”
    Glia 2010, 58,1197-207.
  • Mitoma, J., Fukuda, M. “Core O-glycans required for lymphocyte homing gene knockout mice of core 1 beta1,3-N-acetylglucosaminyltransferase and core 2 N-acetylglucosaminyltransferase”
    Methods Enzymol 2010, 479,257-70.
  • Mitoma, J., Miyazaki, T., Sutton-Smith, M., Suzuki, M., Saito, H., Yeh, J.C., Kawano, T., Hindsgaul, O., Seeberger, P.H., Panico, M., Haslam, S.M., Morris, H.R., Cummings, R.D., Dell, A., Fukuda, M. “The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells”
    GlycoconjJ 2009, 26,511-23.
  • Mitoma, J., Bao, X., Petryanik, B., Schaerli, P., Gauguet, J.M., Yu, S.Y., Kawashima, H., Saito, H., Ohtsubo, K., Marth, J.D., Khoo, K.H., von Andrian, U.H., Lowe, J.B., Fukuda, M. “Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment”
    Nat Immunol 2007, 8,409-18.
  • Mitoma, J., Fukuda, M. “Expression of specific carbohydrates by transfection with carbohydrate modifying enzymes”
    Methods Enzymol 2006, 416,293-304.
  • Higashi, H., Ito, M. “Analysis of sugar chain structures using an endoglycoceramidase”. In: Endo, M., Yamamoto, K., Takagaki, K., Hase, S., eds. Endoglycosidases – Biochemistry, Biotechnology, Application. Tokyo, Heidelberg: Kodansha, Springer; 2006, 227-38.
  • Nakagawa, T., Morotomi, A., Tani, M., Sueyoshi, N., Komori, H., Ito, M. “C18:3-GM1a induces apoptosis in Neuro2a cells: enzymatic remodeling of fatty acyl chains of glycosphingolipids”
    Journal of Lipid Research 2005, 46,1103-12.
  • Kawashima, H., Petryniak, B., Hiraoka, N., Mitoma, J., Huckaby, V., Nakayama, J., Uchimura, K., Kadomatsu, K., Muramatsu, T., Lowe, J.B., Fukuda, M. “N-acetylglucosamine-6-O-sulfotransferases 1 and 2 cooperatively control lymphocyte homing through L-selectin ligand biosynthesis in high endothelial venules”
    Nat Immunol 2005, 6,1096-104.
  • Hwang, Y.H., Tani, M., Nakagawa, T., Okino, N., Ito, M. “Subcellular localization of human neutral ceramidase expressed in HEK293 cells”
    Biochem Biophys Res Commun 2005, 331,37-42.
  • Mitoma, J., Furuya, S., Shimizu, M., Shinoda, Y., Yoshida, K., Azuma, N., Tanaka, H., Suzuki, Y., Hirabayashi, Y. “Mouse 3-phosphoglycerate dehydrogenase gene: genomic organization, chromosomal localization, and promoter analysis”
    Gene 2004, 334,15-22.
  • Kobayashi, M., Mitoma, J., Nakamura, N., Katsuyama, T., Nakayama, J., Fukuda, M. “Induction of peripheral lymph node addressin in human gastric mucosa infected by Helicobacter pylori”
    Proc Natl Acad Sci U S A 2004, 101,17807-12.
  • Higashi, H., Chen, N.H. “Ganglioside/protein kinase signals triggering cytoskeletal actin reorganization”
    Glycoconjugate Journal 2004, 20,49-58.
  • Yasuda, H., Higashi, H., Kudo, Y., Inoue, T., Hata, Y., Mikoshiba, K., Tsumoto, T. “Imaging of calcineurin activated by long-term depression-inducing synaptic inputs in living neurons of rat visual cortex”
    European Journal of Neuroscience 2003, 17,287-97.
  • Mitoma, J., Petryniak, B., Hiraoka, N., Yeh, J.C., Lowe, J.B., Fukuda, M. “Extended core 1 and core 2 branched O-glycans differentially modulate sialyl Lewis X-type L-selectin ligand activity”
    J Biol Chem 2003, 278,9953-61.
  • Chen, N., Furuya, S., Shinoda, Y., Yumoto, M., Ohtake, A., Sato, K., Doi, H., Hashimoto, Y., Kudo, Y., Higashi, H. “Extracellular carbohydrate-signal triggering cAMP-dependent protein kinase-dependent neuronal actin-reorganization”
    Neuroscience 2003, 122,985-95.
  • Chen, N., Furuya, S., Doi, H., Hashimoto, Y., Kudo, Y., Higashi, H. “Ganglioside/calmodulin kinase II signal inducing cdc42-mediated neuronal actin reorganization”
    Neuroscience 2003, 120,163-76.

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